黑木耳单孢杂交菌株筛选与灰色关联度分析  

Screening and grey correlation analysis of monospore hybrid strains of Auricularia auricula

作　　者：吴润泽 苏林贺 曾伟民[1] 张彦龙[1] WU Runze;SU Linhe;ZENG Weimin;ZHANG Yanlong(Engineering Research Center of Agricultural Microbiological Technology,Ministry of Education/Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization in Cold Region/Key Laboratory of Microbiology,College of Heilongjiang Province,College of Life Sciences,Heilongjiang University,Harbin 150080,China)

机构地区：[1]黑龙江大学生命科学学院农业微生物技术教育部工程研究中心/黑龙江省寒地生态修复与资源利用重点实验室/黑龙江省普通高校微生物重点实验室,哈尔滨150080

出　　处：《黑龙江大学自然科学学报》2024年第2期207-217,共11页Journal of Natural Science of Heilongjiang University

基　　金：“一带一路”创新人才交流外国专家项目(G2021055)。

摘　　要：为了提高黑木耳品质、创造兼具优良产量和较高商品性的新品种,选择子实体差异较大的两株黑木耳菌株西藏6号和黑丰作为杂交亲本。采用单孢杂交方法选育优良的杂交子代,测量记录农艺学性状,通过灰色关联度和感官评价进行分析筛选。最终筛选出一株优良的杂交子代菌株a7d9,该菌株每袋干重58.33 g、干湿比13.62、单耳片鲜重5.14 g、子实体厚度1.35 mm、子实体最大直径59.25 mm、生物学效率99.31%、感官评价7.05分。杂交菌株a7d9产量较双亲菌株具有明显优势,农艺学性状加权关联系数要高于双亲菌株,感官评价较好,商品性强,具有开发为新品种的潜力。解决了双亲菌株部分性状较差问题,实现菌株改良,增加黑木耳优良菌株多样性,提高了经济效益。In order to improve the quality of Auricularia auricula and create new varieties with both excellent yield and high commerciality.Two strains of Auricularia auricula,Xizang No.6 and Heifeng,with large differences in fruiting bodies,were selected as hybrid parents.Monospore hybridization method was used to select excellent hybrid progenies,and agronomic traits were measured and recorded.Analysis and screening were done through gray correlation degree and sensory evaluation.Finally,an excellent hybrid progeny strain a7d9 is selected,which has a dry weight of 58.33 g per bag,a dry to wet ratio of 13.62,a fresh weight of 5.14 g per ear,a fruiting body thickness of 1.35 mm,a maximum diameter of 59.25 mm,a biological efficiency of 99.31%,and a sensory evaluation score of 7.05.The hybrid strain a7d9 has obvious advantages in yield compared with its parent,and the weighted correlation coefficient of agronomic traits is higher than that of its parent.It has good sensory evaluation,strong commerciality,and the potential to be developed into a new variety.The problem of poor properties of some parental strains is solved,the strain improvement is realized,the diversity of excellent strains of Auricularia auricula is increased,and the economic benefits are improved.

关 键 词：黑木耳 单孢杂交 农艺学性状 灰色关联度分析 感官评价 

分 类 号：S646.6[农业科学—蔬菜学]