智能手机辅助双探针比色法检测α-葡萄糖苷酶  

Detection ofα-Glucosidase by a Smartphone Assisted Dual-probe Colorimetric Method

作　　者：赵灵芝[1,3] 赵柳 张小清[1] 寇张文 魏世楠 ZHAO Ling-zhi;ZHAO Liu;ZHANG Xiao-qing;KOU Zhang-wen;WEI Shi-nan(Department of Pharmacy,Xi'an Medical University,Xi'an 710021,China;Shaanxi Agricultural Inspection and Testing Center,Xi'an 710003,China;Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province,School of Chemistry and Chemical Engineering,Shaanxi Normal University,Xi'an 710062,China)

机构地区：[1]西安医学院药学院,陕西西安710021 [2]陕西省农业检验检测中心,陕西西安710003 [3]陕西师范大学化学化工学院陕西省生命分析重点实验室,陕西西安710062

出　　处：《分析测试学报》2024年第6期905-912,共8页Journal of Instrumental Analysis

基　　金：陕西省科技厅项目(2022JM-094,2021JM-502);西安医学院杰出人才青年计划配套项目(2021PT08);2022年科研能力提升计划项目(2022NLTS072);陕西省科技厅社发项目(2024SF-YBXM-661);咸阳市科技局社发项目(L2023-ZDYF-SF-023)。

摘　　要：该文以3,3',5,5'-四甲基联苯胺(TMB)和罗丹明B为比色探针,建立了α-葡萄糖苷酶的比色检测方法,并在此基础上基于智能手机策略,通过RGB值对α-葡萄糖苷酶进行检测。结果表明,Fe^(3+)-TMB-罗丹明B显色体系的吸光度与α-葡萄糖苷浓度在20~100 U/L范围内呈线性关系,检出限为3.3 U/L。采用photo⁃shop软件分析样品图像和样品荧光图像的RGB值,其与α-葡萄糖苷酶浓度在10~100 U/L范围内呈线性关系,检出限分别为2.5 U/L和1.2 U/L。该法已成功用于α-葡萄糖苷酶抑制剂阿卡波糖抑制率评估和胎牛血清中α-葡萄糖苷酶浓度的测定。所构建的智能手机辅助检测法为α-葡萄糖苷酶的临床分析和酶抑制剂的快速筛选提供了简便、快捷和准确的方法。This study exploited 3,3',5,5'-tetramethylbenzidine(TMB)and Rhodamine B as chromogenic probes forα-glucosidase concentration analysis via a colorimetric detection method.On this basis,a smartphone assisted strategy was designed to detectα-glucosidase activity by RGB value analysis of images.The results showed that the absorbance of Fe^(3+)-TMB-Rhodamine B system was linearly related toα-glucosidase concentration in the range of 20-100 U/L,and the detection limit was 3.3 U/L.The RGB values of the sample photos and fluorescence photos were analyzed by photo⁃shop,which showed a linear relationship withα-glucosidase activity in the range of 10-100 U/L,the detection limits were 2.5 U/L and 1.2 U/L forα-glucosidase,respectively.The method has been successfully used to detectα-glucosidase inhibitor acarbose and to quantitatively detectα-glucosidase activity in spiked fetal bovine serum.The smartphone assisted detection method provided a simple,quick and accurate method for clinical analysis ofα-glucosidase and rapid screening of enzyme inhibi⁃tors.

关 键 词：Α-葡萄糖苷酶 抗坏血酸 智能手机比色法 3 3' 5 5'-四甲基联苯胺(TMB) 罗丹明B 

分 类 号：O657.3[理学—分析化学] O629.8[理学—化学]