Evaluation of laboratory and environmental exposure systems for protein modification upon gas pollutants and environmental factors  

作　　者：Zhiwei Pan ShiyiWu Qiaoze Zhu Fobang Liu Yongjian Liang Chenglei Pei Haoyu Jiang Yingyi Zhang Senchao Lai 

机构地区：[1]The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters,Ministry of Education,School of Environment and Energy,South China University of Technology,Guangzhou 510006,China [2]Department of Environmental Science and Engineering,Xi’an Jiaotong University,Xi’an 710049,China [3]Guangzhou Sub-branch of Guangdong Ecological and Environmental Monitoring Center,Guangzhou 510006,China [4]State Key Laboratory of Organic Geochemistry,Guangdong Key Laboratory of Environmental Protection and Resources Utilization,and Joint Laboratory of the Guangdong-Hong Kong-Macao Greater Bay Area for the Environment,Guangzhou Institute of Geochemistry,Chinese Academy of Sciences,Guangzhou 510640,China [5]School of Marine Sciences,Sun Yat-sen University,Zhuhai 519082,China

出　　处：《Journal of Environmental Sciences》2024年第9期213-223,共11页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.41975156,41675119)。

摘　　要：Chemical modifications of proteins induced by ambient ozone(O_(3))and nitrogen oxides(NOx)are of public health concerns due to their potential to trigger respiratory diseases.The laboratory and environmental exposure systems have been widely used to investigate their relevant mechanism in the atmosphere.Using bovine serum albumin(BSA)as a model protein,we evaluated the two systems and aimed to reduce the uncertainties of both the reactants and products in the corresponding kinetic study.In the laboratory simulation system,the generated gaseous pollutants showed negligible losses.Ten layers of BSA were coated on the flow tube with protein extraction recovery of 87.4%.For environmental exposure experiment,quartz fiber filter was selected as the upper filter with low gaseous O_(3)(8.0%)and NO_(2)(1.7%)losses,and cellulose acetate filter was appropriate for the lower filter with protein extraction efficiency of 95.2%.The protein degradation process was observed without the exposure to atmospheric oxidants and contributed to the loss of protein monomer mass fractions,while environmental factors(e.g.,molecular oxygen and ultraviolet)may cause greater protein monomer losses.Based on the evaluation,the study exemplarily applied the two systems to protein modification and both showed that O_(3) promotes the protein oligomerization and nitration,while increased temperature can accelerate the oligomerization and increased relative humidity can inhibit the nitration in the environmental exposure samples.The developed laboratory and environmental systems are suitable for studying protein modifications formed under different atmospheric conditions.A combination of the two will further reveal the actual mechanism of protein modifications.

关 键 词：Protein modification Laboratory simulation Environmental exposure OLIGOMERIZATION NITRATION 

分 类 号：R12[医药卫生—环境卫生学] X51[医药卫生—公共卫生与预防医学]