基于数据挖掘技术探讨细胞分裂周期相关蛋白在肝细胞癌中的表达及对预后的影响  

Expression of cell division cycle-associated proteins in hepatocellular carcinoma and its influence on prognosis based on data mining technology

作　　者：姚梦础 徐建波[2] 谢宇 YAO Mengchu;XU Jianbo;XIE Yu(Department of Oncology,Huaian First People’s Hospital,Jiangsu Province,Huaian223300,China;Department of Hepatobiliary Surgery,Huaian First People’s Hospital,Jiangsu Province,Huaian223300,China)

机构地区：[1]江苏省淮安市第一人民医院肿瘤内科,江苏淮安223300 [2]江苏省淮安市第一人民医院肝胆外科,江苏淮安223300

出　　处：《中国医药导报》2024年第10期6-10,共5页China Medical Herald

基　　金：国家自然科学基金青年科学基金资助项目(82203722)。

摘　　要：目的 基于数据挖掘技术研究细胞分裂周期相关蛋白(CDCA)在肝细胞癌(HCC)中的表达特征及其与临床病理分期和预后的关系。方法 通过TCGA数据库获得266例HCC组织及50例正常肝组织样本数据,分析CDCA在HCC与正常肝组织中表达的差异,再以CDCA基因表达中位数为界将HCC组织分为高、低表达组,每组133例。Kaplan-Meier法比较高、低表达组患者总生存期(OS)差异。采用单因素和多因素Cox比例危险回归模型识别独立预后因素。另收集江苏省淮安市第一人民医院2019年6月至2020年6月被病理证实的HCC组织及对应的癌旁组织24例,采用随机数字表法将其分为8组,每组3对,采用免疫组织化学染色检测CDCA在HCC及癌旁组织中表达的差异。结果CDCA1-8在HCC标本中表达量高于正常肝组织(P<0.05)。CDCA1/2/5/6/8高表达组总生存率低于低表达组(P<0.05)。CDCA6(HR=1.51,95%CI:1.29~1.75,P<0.001)、CDCA8(HR=1.69,95%CI:1.08~2.64,P<0.05)、年龄(HR=1.03,95%CI:1.01~1.05,P<0.05)、患者肿瘤大小分期[T3(HR=1.46,95%CI:1.47~4.32,P<0.001)、T4(HR=6.61,95%CI:2.84~15.34,P<0.001)]是HCC OS的独立预测因子。结论 CDCA在HCC组织中表达升高,多数CDCA家族成员表达水平与HCC预后显著相关,其中,CDCA6/8是独立预测指标。Objective To investigate the expression characteristics of cell division cycle-associated proteins(CDCA)in hepatocellular carcinoma(HCC)and their relationship with clinicopathologic staging and prognosis based on data mining technology.Methods The data of 266 HCC tissues and 50 normal liver tissues were obtained by TCGA database.The expression difference of CDCA in HCC tissues and normal liver tissues was analyzed.The median CDCA gene expression level was used to divide HCC tissues into high and low expression group,with 133 cases in each group.Kaplan-Meier method was used to verify the differences in overall survival(OS)of patients between the high and low CDCA expression groups.Univariate and multivariate Cox proportional hazard regression models were used to identify independent prognostic factors.A total of 24 HCC tissues and corresponding paracancer tissues samples confirmed by pathology were collected from the Huaian First People’s Hospital,Jiangsu Province from June 2019 to June 2020 were divided into eight groups by random number table methed,with three pairs in each group.Immunohistochemical staining was performed to detect the difference of CDCA expression in HCC and paracancer tissues.Results The expression of CDCA1-8 HCC samples was higher than that in normal liver tissues(P<0.05).The overall survival rate of CDCA1/2/5/6/8 high expression group was lower than low expression group(P<0.05).CDCA6(HR=1.51,95%CI:1.29-1.75,P<0.001),CDCA8(HR=1.69,95%CI:1.08-2.64,P<0.05),age(HR=1.03,95%CI:1.01-1.05,P<0.05),tumor size staging(T3[HR=1.46,95%CI:1.47-4.32,P<0.001],T4[HR=6.61,95%CI:2.84-15.34,P<0.001])was independent predictor of OS of HCC patients.Conclusion The expression level of CDCA is increased in HCC tissues,and the expression level of most CDCA family members is significantly correlated with the prognosis of HCC patients.CDCA6/8 is an independent predictor of the prognosis of HCC patients.

关 键 词：细胞分裂周期相关蛋白 细胞周期 预后 肝细胞癌 

分 类 号：R735.2[医药卫生—肿瘤]