毛花苷C抑制肝癌HepG2细胞增殖及下调Akt表达  

Lanatoside C inhibits the proliferation of HepG2 cells and down-regulates Akt expression

作　　者：王梦玲 刘敏珍 吴丹[3] 韦建伶 郭昌淇 谭宁[3] WANG Mengling;LIU Minzhen;WU Dan;WEI Jianling;GUO Changqi;TAN Ning(College of Pharmacy,Guilin Medical University,Guilin 541199,China;Graduate School,Guilin Medical University,Guilin 541199,China;College of Basic Medicine,Guilin Medical University,Guilin 541199,China;College of Clinical Medicine,Guilin Medical University,Guilin 541199,China)

机构地区：[1]桂林医学院药学院,桂林541199 [2]桂林医学院研究生院,桂林541199 [3]桂林医学院基础医学院,桂林541199 [4]桂林医学院临床医学院,桂林541199

出　　处：《华夏医学》2024年第2期75-80,共6页Acta Medicinae Sinica

基　　金：国家自然科学基金地区基金项目(82160699);广西壮族自治区教育厅2021年研究生教育创新计划项目(JGY2021138);大学生创新创业训练国家级项目(202110601005,202210601017)。

摘　　要：目的探究毛花苷C对肝癌HepG2细胞增殖的作用及其潜在的分子机制。方法采用毛花苷C处理HepG2细胞,利用细胞增殖实验与克隆形成实验评估毛花苷C对其增殖能力的影响。使用流式细胞仪分析细胞周期的分布。采用Western blot实验检测细胞周期相关蛋白p21、p27、CDK4以及p-Akt和Akt的表达。结果毛花苷C呈浓度依耐性抑制HepG2细胞增殖,与对照组相比,毛花苷C干预后,HepG2细胞克隆形成率明显下降,细胞阻滞于G0/G1期。Western blot检测结果揭示,毛花苷C干预HepG2细胞后,p21和p27蛋白的表达增强,p-Akt和Akt的表达降低。结论毛花苷C能抑制HepG2细胞增殖,诱导细胞周期阻滞,其机制可能与毛花苷C抑制Akt的表达与磷酸化,上调细胞周期调节蛋白p21和p27有关。Objective To observe the effect of Lanatoside C on the proliferation in hepatocellular carcinoma cell HepG2 and to explore its potential molecular mechanism.Methods HepG2 cells were treated with various concentrations of Lanatoside C,and the effect of Lanatoside C on the proliferation of HepG2 cells was evaluated by cell proliferation and clonal formation experiments.Flow cytometry was used to analyze the cell cycle.Western blot assay was used to test the protein expression levels of cell cycle related proteins p21,p27,CDK4,p-Akt and Akt.Results The proliferation of HepG2 cells was significantly inhibited by Lanatoside C in a dose-dependent manner.Compared with the control group,the clonogenesis rate of HepG2 cells was significantly decreased by Lanatoside C.Flow cytometry results showed that Lanatoside C blocked HepG2 cells in G0/G1 phase.Western blot results showed that the protein expressions of p21 and p27 in HepG2 cells were significantly up-regulated by Lanatoside C,and the protein expressions of p-Akt and Akt were significantly down-regulated.Conclusion Lanatoside C inhibites the proliferation and induces the cell cycle arrest in HepG2 cells,which the mechanism may be related to the inhibition of Akt protein expression and phosphorylation induced by Lanatoside C,as well as the up-regulation of cell cycle regulatory proteins p21 and p27 induced by Lanatoside C.

关 键 词：肝癌 毛花苷C 细胞周期 AKT 

分 类 号：R735.7[医药卫生—肿瘤]