人工鱼礁表面分离细菌形成单一生物被膜对厚壳贻贝稚贝附着的影响  

The Effect of Mono-Species Bacterial Biofilms Formed on the Surface of Artificial Reef on Settlement of Plantigrades in Mytilus coruscus

作　　者：杨金龙[1,2,3] 于相冰 胡晓梦 贺楚晗 梁箫 YANG Jinlong;YU Xiangbing;HU Xiaomeng;HE Chuhan;LIANG Xiao(International Research Center for Marine Biosciences,Ministry of Science and Technology,Shanghai Ocean University,Shanghai 201306,China;Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-Culture of Aquaculture Animals,Shanghai 201306,China;Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou),Guangzhou 511458,China)

机构地区：[1]上海海洋大学国家海洋生物科学国际联合研究中心,上海201306 [2]上海市水产动物良种创制与绿色养殖协同创新中心,上海201306 [3]南方海洋科学与工程广东省实验室(广州),广东广州511458

出　　处：《渔业科学进展》2024年第3期224-234,共11页Progress in Fishery Sciences

基　　金：国家重点研发计划(2020YFD0900804);上海市优秀学术带头人计划(20XD1421800);南方海洋科学与工程广东省实验室人才团队引进重大专项(GML2019ZD0402);国家自然科学基金项目(41876159)共同资助。

摘　　要：为探讨存在于人工鱼礁表面的海洋细菌与贝类附着之间的互作关系,本研究从自然海域中的人工鱼礁表面上分离了9株海洋细菌,并分别构建单一细菌生物被膜,探索不同细菌种属形成的生物被膜特性与厚壳贻贝(Mytilus coruscus)稚贝附着之间的关系。结果显示,9株人工鱼礁表面细菌形成的生物被膜对厚壳贻贝稚贝附着的诱导活性存在显著差异,其中,Mesoflavibacter sp.2对稚贝附着的诱导活性最高,Phaeobacter sp.2的诱导活性最低。Sutcliffiella sp.1和Jeotgalibacillus sp.1的细菌密度与诱导活性呈显著正相关,Cytobacillus sp.1和Phaeobacter sp.2的细菌密度与诱导活性呈显著负相关。通过比较分析Mesoflavibacter sp.2和Phaeobacter sp.2生物被膜的蛋白质及多糖含量发现,生物被膜对厚壳贻贝稚贝附着的诱导活性与多糖含量呈显著负相关,与蛋白质含量呈正相关。本研究初步探索了人工鱼礁表面细菌形成的生物被膜对厚壳贻贝稚贝附着的影响,为后续在自然海区进一步解析人工鱼礁表面生物被膜与海洋无脊椎动物附着的互作关系具有重要理论研究意义,同时,对于人工鱼礁表面海洋生物附着机制的研究具有重要的实践价值。Aquaculture is the fastest growing food production industry.The Chinese mariculture industry has made great progress in the past 40 years to become the dominant producer of aquaculture.However,the current deterioration of the water environment and the aggravation of farming diseases poses challenges for the traditional culture model to meet the new requirements of healthy development in marine fishery resources.This problem was addressed using marine ranching.This is a new mariculture model that includes two approaches(artificial reefs and stocking)with the goal of achieving environmental and ecological harmony.Artificial reefs are an important component of this marine ranching model since they can help improve the water environment around the reefs,promote nutrient circulation,provide a suitable habitat for marine organisms,and encourage colonization of the reef surface by sessile organisms.The placement of artificial reefs into seawater may serve as a substrate for bacteria to form a biofilm on the surface.Biofilms play a crucial role in the settlement of many marine invertebrate species.However,limited research was conducted into the relationship between bacteria on the surface of artificial reefs,biofilm formation,and the settlement behavior of Mytilus coruscus.This study placed white acrylic plates and tetrahedral structured artificial reefs in Gouqi Island,Zhoushan City,Zhejiang Province,China(122°46′E;30°43′N).Nine strains of bacteria isolated from the surface of the artificial reefs were used to construct mono-species bacterial biofilms and induce plantigrade settlement of M.coruscus.The aim of the study was to investigate the interactions between marine bacteria and the settlement of M.coruscus on the surface of artificial reefs.The marine biofilms impacted the settlement process of M.coruscus.The bacterial species from the marine biofilm were screened for high and low inducing activity and analyzed for bacterial density,protein,and polysaccharide content to further explore the relationship between

关 键 词：人工鱼礁 厚壳贻贝 稚贝 附着 生物被膜 

分 类 号：S968.3[农业科学—水产养殖]