萝卜叶多酚的超声波辅助提取工艺优化及抗氧化活性研究  

Optimization of Ultrasonic-assisted Extraction Technology and Antioxidant Activity of Polyphenols from Raphanus Sativus Leaves

作　　者：蓝志福[1] LAN Zhifu(College of Continuing Education,Zhangzhou Institute of Technology,Zhangzhou 363000,China)

机构地区：[1]漳州职业技术学院继续教育学院,福建漳州363000

出　　处：《湖北民族大学学报（自然科学版）》2024年第2期158-163,190,共7页Journal of Hubei Minzu University:Natural Science Edition

基　　金：福建省科技特派员项目(MKNH202315)。

摘　　要：为提高萝卜叶资源的利用率,并发掘其潜在应用价值,探究了萝卜叶多酚的超声波辅助提取工艺,并对其抗氧化活性进行了评估。通过在单因素实验基础上引入Box-Behnken响应面法,对萝卜叶多酚的超声波辅助提取工艺进行了优化。通过体外实验的评估,进一步验证了其抗氧化能力。结果表明,在超声温度为68℃、液料比为32 mL/g、乙醇体积分数为63%以及超声时间为31 min的最佳工艺条件下,萝卜叶多酚可以被高效地提取,其提取率达到了60.84 mg/g,且相对误差低至0.73%,充分证实了响应面分析方法优化该提取工艺的有效性和可行性。萝卜叶多酚展现出一定的抗氧化特性,对DPPH自由基、O_(2)^(-)自由基的半抑制质量浓度(half maximal inhibitory concentration,IC_(50))分别为78.91、403.55 mg/L,为进一步研发其作为天然植物源抗氧化剂提供了参考。In order to improve the utilization rate of Raphanus sativus leaves resources and explore their potential application value,the ultrasonic-assisted extraction technology for polyphenols from Raphanus sativus leaves was explored and its antioxidant activity was evaluated.By introducing Box-Behnken response surface method on the basis of single factor experiment,the ultrasonic-assisted extraction process of polyphenols from Raphanus sativus leaves was optimized,and its antioxidant capacity was further verified by the evaluation of in vitro experiments.The results showed that under the optimum conditions of ultrasonic temperature of 68℃,liquid-solid ratio of 32 mL/g,ethanol volume fraction of 63%and ultrasonic time of 31 min,the extraction yield of polyphenols from Raphanus sativus leaves was 60.84 mg/g,and the relative error was as low as 0.73%,which fully confirmed the effectiveness and feasibility of response surface analysis to optimize the extraction process.Polyphenols from Raphanus sativus leaves showed certain antioxidant properties,and the half maximum inhibitory concentration(IC_(50))of DPPH and O_(2)^(-)radical were 78.91 mg/L and 403.55 mg/L,respectively,which provided reference for further research and development of antioxidants from natural plants.

关 键 词：萝卜叶 多酚 超声波辅助提取 单因素实验 响应面实验 抗氧化 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程] R93[轻工技术与工程—食品科学与工程] O629[医药卫生—生药学]