两歧双歧杆菌B11调控肠道屏障的体外研究  

Study on the regulation of intestinal barrier by Bifidobacterium bifidum B11 in vitro

作　　者：贾晓蒙 路江浩 李思童 李旭阳 贾洪利 梁丛丛 霍文敏 郭润晴 杨玲 JIA Xiaomeng;LU Jianghao;LI Sitong;LI Xuyang;JIA Hongli;LIANG Congcong;HUO Wenmin;GUO Runqing;YANG Ling(Research and Development Center,Hebei Inatural Biotech Co.Ltd.,Shijiazhuang 050000,China)

机构地区：[1]河北一然生物科技股份有限公司研发中心,石家庄050000

出　　处：《中国乳品工业》2024年第5期11-15,共5页China Dairy Industry

基　　金：河北省农业高质量发展关键共性技术攻关专项项目(21322804D);石家庄市高层次科技创新创业人才(06202103)。

摘　　要：文章以肠道菌群体外批量发酵系统与HT-29细胞相结合,分析了两歧双歧杆菌(Bifidobacterium bifidum)B11对肠道机械屏障的调控及对产肠毒素性大肠杆菌(Enterotoxigenic E.coli,ETEC)的拮抗作用,通过对巨噬细胞RAW264.7影响,探讨B11对免疫屏障的调控。结果表明,两歧双歧杆菌B11可在体外发酵条件下拮抗ETEC对HT-29的损伤,提高HT-29黏蛋白MUC2和MUC5AC、紧密连接蛋白ZO-2、Claudin-1和Occudin的mRNA相对表达率至3.78、1.75、4.86、4.06和3.29。经过B11菌悬液处理RAW264.7细胞的相对增殖率提升(17.48±2.49)%,吞噬率提升(21.63±9.85)%,促进了细胞因子IL-12和TNF-α的分泌(P<0.05)。可知,两歧双歧杆菌B11能够增强肠道机械屏障和免疫屏障。In this study,the regulation of Bifidobacterium bifidum B11 on intestinal mechanical barrier and its antagonistic effect on Enterotoxigenic E.coli(ETEC)were analyzed by combining the in vitro batch fermentation system of intestinal flora with HT-29 cells.And its regulation mechanism on immune barrier was investigated through its effect on macrophages RAW264.7.The results showed that Bifidobacterium bifidum B11 could antagonize the damage of ETEC to HT-29 under the condition of in vitro fermentation,and increase the relative mRNA expression rate of MUC2,MUC5AC,tight junction protein ZO-2,Claudin-1 and Occudin to 3.78,1.75,4.86,4.06 and 3.29.The relative proliferation rate of RAW264.7 cells treated with B11 suspension increased by(17.48±2.49)%,the phagocytosis rate increased to(121.63±9.85)%,and the secretion of cytokines IL-12 and TNF-αwere promoted(P<0.05).In conclusion,Bifidobacterium bifidum B11 can strengthen the intestinal mechanical barrier and immune barrier,and has the potential to further develop as probiotic.

关 键 词：两歧双歧杆菌 肠道屏障 产肠毒素性大肠杆菌 免疫 益生菌 

分 类 号：Q939.9[生物学—微生物学]