口腔黏膜伤口愈合过程的基因表达分析  

Gene expression profiling of the wound healing process in the oral mucosa

作　　者：郑保柱 吕梦皓 赵莹初 张凤秋[1] 徐骏疾[1] 刘怡[1] ZHENG Baozhu;LYU Menghao;ZHAO Yingchu;ZHANG Fengqiu;XU Junji;LIU Yi(Department of Periodontics&Oral Medicine,Beijing Stomatological Hospital,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京口腔医院牙周病科,北京100050

出　　处：《口腔生物医学》2024年第3期131-139,146,共10页Oral Biomedicine

基　　金：国家优秀青年科学基金(82122015)。

摘　　要：目的:分析口腔黏膜伤口愈合过程中的基因表达特征,为深入研究口腔黏膜愈合过程提供依据。方法:本研究通过剥脱小鼠上颚第一磨牙至第三磨牙全层软硬颚黏膜组织构建小鼠腭部黏膜伤口模型,提取0、1、3、5、7 d创伤后黏膜伤口组织进行转录组RNA测序,通过数据分析,构建口腔黏膜伤口愈合各阶段的基因表达谱特征。结果:GO富集分析显示,黏膜伤口愈合第1天免疫反应活跃(P<0.05),第3天伤口愈合及肽酶和内肽酶相关的通路显著上调(P<0.05),第5天免疫反应及含胶原蛋白的细胞外基质(ECM)相关通路显著上调(P<0.05),第7天免疫反应通路相关基因及含胶原外基质相关基因表达活跃(P<0.05)。结论:黏膜伤口愈合的前3 d炎症阶段以白细胞及免疫球蛋白介导的免疫反应为主,第3天伤口愈合开始进行再上皮化,在第5天之后白细胞及免疫球蛋白介导的免疫反应再次活跃,且伤口愈合中含胶原蛋白的ECM成分上调表达可能与黏膜伤口下纤维改建相关。Objective:This article is to make a comprehensive analysis of oral mucosal wound healing process at the genetic level.Methods:In this study,a mouse palatal mucosal wound model was constructed by exfoliating the whole layer of soft and hard palatal mucosal tissues from the first to the third molar in the upper jaw of mice,and by extracting the mucosal wound tissues on 0,1,3,5,and 7 days post-traumatization and sequencing the transcriptome RNA,we constructed the characteristics of the gene expression profiles of the various stages of the oral mucosal wound healing through the analysis of the data.Results:Differential genes on days 1,3,5,and 7 of mucosal wound healing were compared with those of normal maxillary mucosa and analyzed by GO enrichment.The immune response was active on day 1 of mucosal wound healing(P<0.05),wound healing and peptidases and endopeptidases-related pathways were significantly up-regulated on day 3(P<0.05),immune response and collagen-containing extracellular matrix(ECM)-related pathways were significantly up-regulated on day 5(P<0.05),and expression of genes related to the immune response pathway and collagen-containing extracellular matrix-related genes was active on day 7(P<0.05).Conclusions:The inflammatory phase of mucosal wound healing is dominated by leukocyte-mediated and immunoglobulin-mediated immune responses during the first three days of wound healing,re-epithelialization begins on the third day of wound healing,and leukocyte-mediated and immunoglobulin-mediated immune responses are active again on the fifth day and beyond,and the expression of collagen-containing components of the ECM upregulated in the healing of wounds may be associated with mucosal wound healing.

关 键 词：口腔黏膜 伤口愈合 转录组测序 

分 类 号：R781.5[医药卫生—口腔医学]