苦荞种质资源萌发期抗旱性综合评价  

Comprehensive evaluation of drought resistance of Tartary buckwheat(Fagopyrum tataricum)germplasm resources at germination stage

作　　者：赵少迪 胡传伟 张玉莲 吕娜 曹利萍 马名川 刘璋 刘龙龙[1] 张丽君[1] ZHAO Shaodi;HU Chuanwei;ZHANG Yulian;LV Na;CAO Liping;MA Mingchuan;LIU Zhang;LIU Longlong;ZHANG Lijun(Agricultural Gene Resources Research Center of Shanxi Agricultural University,Taiyuan,Shanxi 030031,China;College of Agriculture,Shanxi Agricultural University,Jinzhong,Shanxi 030801,China;Institute of Economic Crops,Shanxi Agricultural University,Taiyuan,Shanxi 030031,China)

机构地区：[1]山西农业大学农业基因资源研究中心,山西太原030031 [2]山西农业大学农学院,山西晋中030801 [3]山西农业大学经济作物研究所,山西太原030031

出　　处：《干旱地区农业研究》2024年第3期13-21,共9页Agricultural Research in the Arid Areas

基　　金：中央引导地方科技发展资金项目(YDZJSX2021A031);山西省基础研究计划项目(202103021224159);山西省现代产业技术体系建设(2023CYJSTX03-11);财政部和农业农村部:国家现代农业产业技术体系(CARS-07-A-2);山西农业大学博士项目(2021BQ38);山西省高等学校科技创新项目(2022L092)。

摘　　要：以PEG-6000为渗透剂对110份苦荞种质资源进行模拟干旱处理,研究了苦荞种子在0、5%、10%、15%、20%的PEG-6000溶液胁迫下的萌发情况,分析了种子的相对发芽势、相对发芽率、相对萌发指数、相对胚根长和相对胚根粗和萌发耐旱指数的变化。结果表明:15%PEG-6000可以作为苦荞种子萌发期耐旱性鉴定的适宜浓度;通过主成分分析法确定了相对发芽势、相对发芽率、相对萌发指数为关键指标,利用隶属函数结合权重分析法得到了萌发期苦荞种质资源耐旱性的综合评价值D。根据D值排序,筛选鉴定到耐旱型5份、较耐旱型28份、中间型48份、敏感型29份。通过聚类分析将110份苦荞种质资源划分为5个亚群,亚群Ⅰ是一类耐旱能力较强的亚群,亚群Ⅱ和亚群Ⅲ为中度耐旱亚群,亚群Ⅳ和亚群Ⅴ为典型的旱敏感亚群。110 Tartary buckwheat germplasm resources were treated with PEG-6000 as an osmotic agent to simulate drought treatment.The germination of Tartary buckwheat seeds under 0,5%,10%,15%and 20%PEG-6000 solution stress were studied.The changes of relative germination potential,relative germination rate,relative germination index,relative radicle length and relative radicle diameter and germination drought tolerance index of seeds were analyzed.The results showed that 15%PEG-6000 was the suitable concentration for the identification of drought tolerance of Tartary buckwheat seeds during germination.The relative germination potential,relative germination rate and relative germination index were determined as the key indexes by principal component analysis.The comprehensive evaluation value D of drought tolerance of Tartary buckwheat germplasm resources at germination stage was obtained by membership function combined with weight analysis.According to the D value,5 drought-tolerant types,28 drought-tolerant types,48 intermediate types and 29 sensitive types were screened and identified.110 Tartary buckwheat germplasm resources were divided into 5 subgroups by cluster analysis.Subgroup I was a subgroup with strong drought tolerance,subgroup II and subgroup III were moderate drought tolerance subgroups,and subgroup IV and subgroup V were typical drought sensitive subgroups.

关 键 词：苦荞 种质 PEG胁迫 萌发期 抗旱性评价 

分 类 号：S517[农业科学—作物学]