基于RNA-Seq技术对不同品种猪排卵前卵泡差异表达基因的筛选与注释  

Screening and Annotation of Differentially Expressed Genes in Preovulatory Follicles of Different Breeds of Pigs Based on RNA-Seq Technology

作　　者：刘林清[1] 李庆岗[1] 苏世广[1] 周梅 张威 王重龙[1] LIU Lin-qing;LI Qing-gang;SU Shi-guang(Institute of Animal Husbandry and Veterinary Medicine,Anhui Academy of Agricultural Sciences/Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety Engineering,Hefei,Anhui 230031)

机构地区：[1]安徽省农业科学院畜牧兽医研究所/畜禽产品安全工程安徽省重点实验室,安徽合肥230031

出　　处：《安徽农业科学》2024年第11期76-79,共4页Journal of Anhui Agricultural Sciences

基　　金：2022年度畜禽产品安全工程安徽省重点实验室青年人才托举工程创新引导资金(XMT2022-09);安徽省财政农业种质资源保护与利用资金项目;安徽省学术和技术带头人后备人选项目(2022H300);安徽省科技特派团项目;安徽省生猪产业技术体系项目(AHCYJSTX-05-12,AHCYJSTX-05-23)。

摘　　要：[目的]筛选淮猪和大约克猪排卵前卵泡组织差异表达基因。[方法]采集淮猪和大约克猪各3头的排卵前卵泡,提取RNA,个体独立建库。采用RNA测序技术以及GO和pathway方法对所获序列进行显著性富集分析。[结果]测序后,获得的淮猪clean reads数分别为85958912、91701082、84448828,其中有效Reads数分别达到98.39%、98.59%、96.04%;获得的大约克猪clean reads数分别为87914166、80480542、85994612,其中有效reads数分别达到98.61%、96.95%、98.80%,测序饱和度良好(证明测序数据真实可靠)。结果表明,筛选到上调基因67个,下调基因54个。GO和Pathway分析发现,差异表达基因富集在繁殖过程、生长过程和代谢过程及WNT信号通路、卵巢类固醇生成通路和类固醇生物合成通路等。[结论]获得了猪排卵前卵泡基因表达谱,并筛选到与繁殖性状相关的关键候选基因。[Objective]This study aimed to find differentially expressed genes in preovulatory follicles of Huai pig and Yorkshire pig.[Method]This study collected preovulatory follicles from three Huai pig and three Yorkshire pig,respectively,extracted RNA and established individual independent libraries.RNA-Seq technology was used to conduct high-throughput Transcriptome sequencing of the sample library,and the obtained sequences were analyzed for GO and pathway significance enrichment.[Result]The number of clean reads obtained from Huai pigs was 85958912,91701082,84448828,with effective reads reaching 98.39%,98.59%,and 96.04%,respectively;the number of clean reads obtained from the large Yorkshire pig was 87914166,80480542,85994612,with effective reads reaching 98.61%,96.95%,and 98.80%,respectively.The high saturation degree of sequencing demonstrated that the data were realistic and reliable.The results showed that 67 upregulated genes and 54 down regulated genes were screened.GO and Pathway analysis found that differentially expressed genes were enriched in the reproductive,growth,and metabolic processes,as well as the WNT signaling pathway,ovarian steroid production pathway,and steroid biosynthesis pathway.[Conclusion]This study obtained the gene expression profiling of porcine preovulatory follicles and identified novel candidate genes for reproductive traits.

关 键 词：淮猪 大约克猪 RNA-SEQ 差异表达基因 

分 类 号：S828[农业科学—畜牧学]