浙江省香椿溃疡病病原菌的分离和鉴定  

Isolation and Identification of the Pathogen Causing Canker Disease of Toona sinensis in Zhejiang Province

作　　者：耿显胜[1] 舒金平[1] 刘莹 刘军[1] Geng Xiansheng;Shu Jinping;Liu Ying;Liu Jun(Research Institute of Subtropical Forestry,Chinese Academy of Forestry Hangzhou 311400)

机构地区：[1]中国林业科学研究院亚热带林业研究所,杭州311400

出　　处：《林业科学》2024年第5期151-157,共7页Scientia Silvae Sinicae

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2020SZ004)。

摘　　要：[目的]分离和鉴定引起浙江省香椿溃疡病的病原菌,揭示香椿溃疡病的病因,为该病害的科学防控提供理论依据。[方法]观察香椿溃疡病的田间症状,使用常规组织分离法分离病原菌,利用形态学结合分子生物学方法对病原菌进行物种鉴定,采用柯赫氏法则验证分离菌株的致病性。[结果]1)从感病香椿样品中分离到2株病原菌;病原菌的培养性状、有性世代和无性世代的形态特征与假毛丛赤壳菌一致。2) LSU rDNA、ITS rDNA和tef1-α基因序列分析以及多基因系统发育分析表明,XCKY2菌株的LSU rDNA、ITS rDNA和tef1-α基因片段与假毛丛赤壳菌的同源性均为100.00%;XCKY1和XCKY2菌株与11株假毛丛赤壳菌聚为一支,贝叶斯后验概率为0.94。3)致病性测定结果表明,假毛丛赤壳菌的菌饼和分生孢子悬浮液均能引起健康香椿苗发病,从发病香椿苗组织中也能再分离到假毛丛赤壳菌,由此确定假毛丛赤壳菌为香椿溃疡病的病原菌。[结论]从浙江省感病香椿样品上分离到2株假毛丛赤壳菌,经鉴定为引起香椿溃疡病的病原菌。本研究首次报道假毛丛赤壳菌是香椿溃疡病病原菌。【Objective】This study isolated and identified the pathogen causing canker disease of Toona sinensis in Zhejiang Province,to reveal the etiology of the disease and provide a theoretical basis for controlling Chinese toon canker disease.【Method】The symptoms of T.sinensis canker disease under natural conditions were observed.The conventional tissue isolation approach was used to isolate the causal pathogen from infected tissues.The morphological traits and molecular methods were used to identify the pathogen species.The pathogenicity of the isolates was verified according to Koch’s postulates.【Result】1)Two strains of pathogenic fungi were isolated from the infected tissues of T.sinensis,XCKY1 and XCKY2.The culture and morphological characteristics of the teleomorph and anamorph of the pathogen were consistent with those of Nectria pseudotrichia.2)The LSU rDNA,ITS rDNA,and tef1-αgene fragments of the XCKY2 strain were all 100.00%homologous to those of N.pseudotrichia.The XCKY1 and XCKY2 strains clustered with 11 N.pseudotrichia strains with a Bayesian posterior probability of 0.94.3)The pathogenicity test result showed that both fungal agar plugs and conidial suspensions of N.pseudotrichia caused necrotic lesions on healthy T.sinensis seedlings,and N.pseudotrichia was re-isolated from the necrotic tissue.Thus,it was confirmed that N.pseudotrichia is the causal agent of T.sinensis canker disease.【Conclusion】Two strains of N.pseudotrichia isolated from infected tissues of T.sinensis in Zhejiang Province have been identified as the pathogen causing Chinese toon canker disease.This is the first report of N.pseudotrichia causing canker disease in T.sinensis.

关 键 词：香椿 溃疡病 病原真菌 致病性 系统发育分析 柯赫氏法则 

分 类 号：S736.15[农业科学—林学]