石碌含笑花苞和叶片的精油化学成分分析及α-葡萄糖苷酶抑制活性研究  

Analysis of the chemical composition of the essential oils from floral buds and leaves of Michelia shiluensis and their inhibitory activity againstα-glucosidase

作　　者：钱昌明 吴文滔 陈健[1] 宋希强[2] 赵莹[2] QIAN Changming;WU Wentao;CHEN Jian;SONG Xiqiang;ZHAO Ying(School of Food Science and Engineering,Hainan University,Haikou,Hainan 570228,China;Key Laboratory of fcMinistry of Education for Genetics and Germplasm Innovation of Tropical Special Trees and Ornamental Plants/Hainan Key Laboratory for Biology of Germplasm Resources of Tropical Special Ornamental Plants,School of Forestry,Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]海南大学食品科学与工程学院,海口570228 [2]海南大学林学院/热带特色林木花卉遗传与种质创新教育部重点实验室/海南省热带特色花木资源生物学重点实验室,海口570228

出　　处：《热带生物学报》2024年第3期290-298,共9页Journal of Tropical Biology

基　　金：海南省重点研发项目(ZDYF2020099、ZDYF2022XDNY179);中国热带农业科学院基本科研业务费专项资金(1630052021015)。

摘　　要：本研究以石碌含笑(Michelia shiluensis)为原材料,利用水蒸气蒸馏法提取石碌含笑花苞和叶片的精油,经过处理后利用气相色谱-质谱联用技术(GC-MS)测定其挥发油的组成成分。GC-MS分析结果表明,两种精油的主要成分均为倍半萜类化合物。从花苞中共鉴定出39个化学成分,其中相对含量最高的3个分别为β-红没药烯、α-姜黄烯及β-榄香烯。从叶片中共鉴定出31个化学成分,主要有愈创木醇、异愈创木醇、(Z,Z,Z)-1,8,11,14-十七碳四烯等。醇类和烯烃类成分的相对含量是区分石碌含笑花苞和叶片精油的重要指标。首次分析了石碌含笑精油成分并测定含笑属植物精油对α-葡萄糖苷酶活性。当质量浓度为3 g·L^(-1)时,叶片和花苞对α-葡萄糖苷酶活性的抑制率分别为87.13%、72.27%,IC50分别为(0.73±0.04)g·L^(-1)、(1.77±0.07)g·L^(-1)。石碌含笑精油具有较强的抑制α-葡萄糖苷酶活性的作用,可作为降血糖功效因子加以开发利用,同时为石碌含笑资源高值化利用提供理论依据。Essential oil was extracted from floral buds and leaves of Michelia shiluensis(M.shiluensis)by steam distillation.After treatment,the components of the essential oil were determined by gas chromatography-mass spectrometry(GC-MS).The GC-MS analysis showed that the main components of the two essential oils were sesquiterpenes.A total of 39 chemical components were identified from the essential oil of floral buds,of which three,β-bisabolene,α-curcumene andβ-elemene,were the highest in relative content.A total of 31 chemical constituents were identified from the essential oil of leaves,including guaiacol,isoguaiacol,(Z,Z,Z)-1,8,11,14-heptadecatetraene,and so on.The relative content of alc cohols and olefins is an important index to distinguish between the essential oils of the floral buds and the leaves.The components of M.shiluensis essential oil were analyzed for the first time,and the inhibitory activity of M.shiluensis essential oil onα-glucosidase was determined.When the mass concentration was 3 g·L^(-1),the essential oils of the leaves and floral buds had an inhibition rate of 87.13%and 72.27%againstα-glucosidase activity,and their IC50 was 0.73±0.04 g·L^(-1) and 1.77±0.07 g·L^(-1) ,respectively.M.shiluensis essential oil has a strong inhibitory effect onα-glucosidase activity,and can be developed and used as a hypoglycemic efficacy factor,which provides a theoretical basis for value-added utilization of M.shiluensis resources.

关 键 词：石碌含笑 精油 气相色谱-质谱联用技术 化学成分 

分 类 号：Q946.85[生物学—植物学]