固相萃取结合液质联用法快速筛查和分析生活饮用水中的56种抗生素  

Rapid Screening and Determination of 56 Antibiotics in Drinking Water by Solid-Phase Extraction Combined with UPLC-MS/MS

作　　者：陈琨[1] 钟格梅[1] 韦日荣[1] 黄江平[1] 雷宁生[1] 廖艳华[1] CHEN Kun;ZHONG Gemei;WEI Rirong;HUANG Jiangping;LEI Ningsheng;LIAO Yanhua(Guangxi Center for Disease Control and Prevention,Nanning 530028,China)

机构地区：[1]广西壮族自治区疾病预防控制中心,广西南宁530028

出　　处：《食品安全导刊》2024年第13期39-46,50,共9页China Food Safety Magazine

基　　金：广西医药卫生计划课题(Z2015448)。

摘　　要：目的:建立生活饮用中56种抗生素的固相萃取-液相色谱-串联质谱分析方法。方法:将生活饮用水通过大容量采样管连接到固相萃取柱上净化,用C18柱梯度洗脱分离目标物,以乙腈和0.1%甲酸水为流动相,经电喷雾离子源电离,多反应监测模式串联质谱检测。结果:56种抗生素的最低检测浓度为0.1~2.0μg·L^(-1),在其线性范围内的相关系数均大于0.995,3个浓度水平的加标回收率为63.3%~113.0%,相对标准偏差在3.1%~8.3%。结论:该方法前处理简单、快速,适用于生活饮用水中多种抗生素的快速筛查和定量检测。Objective:To develop a method for determination of 56 antibiotics in drinking water by solid-phase extraction and UPLC-MS/MS.Method:Drinking water is connected to the solid phase extraction column for purification through a large-capacity sampling tube,target object were separated on C18 with gradient elution using acetonitrile-0.10%formic acid as mobile phase and detected by MS/MS using positive electrospray ionization under the multiple reaction monitoring.Result:The minimum detection of 56 antibiotics was 0.1~2.0μg·L^(-1).The correlation coefficients in its linear range are over 0.995.The recovery rates of the 3 concentration levels were 63.3%~113.0%with the relative standard deviations of 3.1%~8.3%.Conclusion:The method has simple and rapid pretreatment,and is suitable for rapid screening and quantitative detection of multiple antibiotics in drinking water.

关 键 词：超高效液相色谱-串联质谱 固相萃取 生活饮用水 大容量采样管 

分 类 号：TU991.21[建筑科学—市政工程] O657.63[理学—分析化学]