超高效液相色谱-串联质谱法同时测定水果中3种苯并咪唑类杀菌剂  

Determination of Three Benzimidazole Fungicides in Fruits by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry

作　　者：别怀周 郑连港 BIE Huaizhou;ZHENG Liangang(Weifang Harrens-HC Testing Technology Co.,Ltd.,Weifang 261000,China;Shandong Shibiao Testing Technology Co.,Ltd.,Weifang 261000,China)

机构地区：[1]潍坊海润华辰检测技术有限公司,山东潍坊261000 [2]山东世标检测技术有限公司,山东潍坊261000

出　　处：《食品安全导刊》2024年第5期113-115,120,共4页China Food Safety Magazine

摘　　要：目的:构建一种超高效液相色谱-串联质谱法(Ultra Performance Liquid Chromatography Tandem Mass Spectrometry,UPLC-MS/MS)同时测定水果中3种苯并咪唑类杀菌剂的方法。方法:试样匀浆后用1%甲酸-乙腈超声提取,提取液经乙二胺-N-丙基硅烷和无水硫酸镁净化,采用UPLC-MS/MS测定。结果:3种杀菌剂线性关系良好,相关系数R2均大于0.999,定量限在0.05~1.16μg·kg^(-1),选定3个浓度进行添加回收验证实验,回收率为87.1%~103.2%,相对标准偏差为1.4%~6.5%。结论:该方法简捷、准确,可满足水果中3种苯并咪唑杀菌剂的快速检测与确证分析要求。Objective:To develop a method for simultaneous determination of three benzimidazole fungicides in fruits by ultra-high performance liquid chromatography-tandem mass spectrometry.Method:The samples were homogenized,followed by ultrasonic extraction with 1%formic acid-acetonitrile,the extraction solution was purified by primary secondary amine and anhydrous magnesium sulfate,and detected by UPLC-MS/MS.Result:The linearity of each fungicide was good,with linear correlation coefficients were greater than 0.999,and the limits of detection were 0.05~1.16μg·kg^(-1),three concentrations were selected for the addition and recovery experiment,and the recovery rate of spiked samples were 87.1%~103.2%,and the relative standard deviations were 1.4%~6.5%.Conclusion:The method is simple,convenient and accurate,and is suitable for the rapid detection and confirmatory analysis of three benzimidazole fungicides in fruits.

关 键 词：苯并咪唑类杀菌剂 水果 超高效液相色谱-串联质谱法 

分 类 号：O657.63[理学—分析化学] TS255.7[理学—化学]