金属有机框架富集-高效液相色谱法测定预包装果蔬制品中双酚A含量  

Determination of Bisphenol A in Prepackaged Fruits and Vegetables by Metal-Organic Framework Enrichment and High-Performance Liquid Chromatography

作　　者：刘镇 曲丽洁[1] LIU Zhen;QU Lijie(Key Laboratory of Quality&Safety Analysis-Testing for Agro-Products and Food of Hebei Province,Hebei North University,Zhangjiakou 075000,China)

机构地区：[1]河北省农产品食品质量安全分析检测重点实验室河北北方学院,河北张家口075000

出　　处：《食品安全导刊》2024年第5期116-120,共5页China Food Safety Magazine

基　　金：河北省高等学校科学技术研究项目(QN2020402);河北北方学院引育博士科研基金项目(BSJJ202215)。

摘　　要：目的:建立金属有机框架富集-高效液相色谱法测定预包装果蔬制品中双酚A的方法。方法:基于QuEChERS处理技术,通过优化提取和净化条件,利用金属有机框架材料富集果蔬样品中的双酚A,采用高效液相色谱法测定。结果:在优化条件下,双酚A在10~1000μg·L^(-1)具有良好的线性关系,相关系数(R^(2))大于0.9990,检出限为0.1μg·kg^(-1),定量限为0.3μg·kg^(-1)。不同果蔬基质的加标回收率在80.5%~105.2%,相对标准偏差为9.6%。不同果蔬基质效应对定量检测影响不显著(|ME|<10%)。结论:该方法灵敏度高,不需要内标和固相萃取装置,操作简便,可满足果蔬中双酚A的检测需求,为食品中双酚A的检测提供了一种经济、便捷的新思路。Objective:To establish a method for the determination of bisphenol A(BPA)in pre-packaged fruits and vegetables using metal-organic framework enrichment and high-performance liquid chromatography(HPLC).Method:Based on the QuEChERS sample preparation technique,the extraction and purification conditions were optimized.Metal organic frameworks were used to enrich BPA from fruit and vegetable samples,which were then analyzed by HPLC.Result:Under optimized conditions,BPA exhibited good linearity in the range of 10~1000μg·L^(-1),with a correlation coefficient(R^(2))greater than 0.9990,a limit of detection of 0.1μg·kg^(-1),and a limit of quantification of 0.3μg·kg^(-1).The recovery rate of different fruit and vegetable matrices ranged from 80.5%to 105.2%,and the relative standard deviation was 9.6%.The effect of different fruit and vegetable matrices on quantitative detection was not significant(|ME|<10%).Conclusion:The approach exhibits remarkable sensitivity,does not require internal standards or solid-phase extraction devices,is simple to operate,and can meet the detection requirements of BPA in fruits and vegetables,providing an economical and convenient new approach for the detection of BPA in food.

关 键 词：双酚A 高效液相色谱 QUECHERS 金属有机框架 吸附 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程] O657.72[轻工技术与工程—食品科学与工程]