大苞鞘石斛组培扩繁研究  

Research on tissue culture and propagation of Dendrobium wardianum Warner

作　　者：曾丽红 王凤兰[1,2] 张永强 艾潇潇 温双 肖子航 陈秀哲 ZENG Lihong;WANG Fenglan;ZHANG Yongqiang;AI Xiaoxiao;WEN Shuang;XIAO Zihang;CHEN Xiuzhe(College of Horticulture and Landscape Architecture,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;Zhonghua Modern Agriculture Research Institute,Huadu District,Guangzhou 510800,China;Guangzhou Greening Co.,Ltd,Guangzhou 510665,China)

机构地区：[1]仲恺农业工程学院园艺园林学院,广东广州510225 [2]广州市花都区仲花现代农业研究院,广东广州510800 [3]广州市绿化有限公司,广东广州510665

出　　处：《仲恺农业工程学院学报》2024年第2期66-71,共6页Journal of Zhongkai University of Agriculture and Engineering

基　　金：广州市基础研究计划基础与应用基础研究项目(202201011833);广东省现代农业产业技术体系花卉创新团队项目(2022KJ121).

摘　　要：以大苞鞘石斛(Dendrobium wardianum Warner)无菌苗为试材,比较不同培养基组合对大苞鞘石斛组培苗生长的效果.结果表明:最适合大苞鞘石斛类原球茎(Protocorm-Like Bodies,PLBs)增殖的培养基是MS+2.0 mg/L 6-BA+1.0 mg/L NAA+20.0 g/L Su,最适合其丛生芽增殖的培养基是MS+0.3 mg/L NAA+30.0 g/L Su+1.0 g/L活性碳,最适合其丛生芽生根的培养基是1/2 MS+1.0 mg/L 6-BA+0.5 mg/L NAA+30.0 g/L Su+2.5 g/L香蕉粉+1.0 g/L活性碳.The effects of different media combinations on the growth of Dendrobium wardianum Warner tissue culture seedlings were compared using the sterile seedlings of Dendrobium wardianum Warner as the test material.The results showed that the most suitable medium for the protocorm-like bodies(PLBs)of Dendrobium wardianum Warner was MS+2.0 mg/L 6-BA+1.0 mg/L NAA+20.0 g/L Su,the most suitable medium for the proliferation of clustered buds was MS+0.3 mg/L NAA+30.0 g/L Su+1.0 g/L activated carbon,and the most suitable medium for rooting tufted buds was 1/2 MS+1.0 mg/L 6-BA+0.5 mg/L NAA+30.0 g/L Su+2.5g/L banana flour+1 g/L activated carbon.

关 键 词：大苞鞘石斛(Dendrobium wardianum Warner) 组织培养 培养基 外源激素 添加物 

分 类 号：S682.31[农业科学—观赏园艺]