混合有机磷阻燃剂污染物的微生物降解特性及途径  

The degradation characteristics and pathways of organophosphorus flame retardants mixtures by Bacillus pacificus

作　　者：罗雅嫣 汤蕙齐 李翼远 欧阳浩民 黄鸿 陈烁娜[1,3] 仇荣亮 LUO Ya-yan;TANG Hui-qi;LI Yi-yuan;OUYANG Hao-min;HUANG Hong;CHEN Shuo-na;QIU Rong-liang(College of Natural Resource and Environment,South China Agricultural University,Guangzhou 510642,China;Heyuan Branch,Guangdong Laboratory for Lingnan Modern Agriculture Project,Heyuan 517000,China;Guangdong Provincial Key Laboratory of Agricultural&Rural Pollution Abatement and Environmental Safety,Guangzhou 510642,China)

机构地区：[1]华南农业大学资源环境学院,广东广州510642 [2]岭南现代农业科学与技术广东省实验室河源分中心,广东河源517000 [3]广东省农业农村污染治理与环境安全重点实验室,广东广州510642

出　　处：《中国环境科学》2024年第6期3434-3441,共8页China Environmental Science

基　　金：广东省自然科学基金资助项目(2023A1515030284);国家自然科学基金资助项目(42307283);广东省重点领域研发计划项目(2023B0202020001);河源市科技计划项目(河科2021001);岭南现代农业科学与技术广东省实验室河源分中心自主科研项目(DT20220016)。

摘　　要：为了获得更多高效降解有机磷阻燃剂(OPFRs)的菌种资源,探明OPFRs微生物降解途径和作用机制,本研究以一株从广州某污水处理厂剩余污泥中筛选获得的对多种典型OPFRs具有降解能力的太平洋芽孢杆菌(Bacillus pacificus)为研究材料,选取环境中检出率高的磷酸三(2-氯乙基)酯(TCEP)、磷酸三(2-氯丙基)酯(TCPP)、磷酸三甲苯酯(TCP)、磷酸三苯酯(TPhP)作为OPFRs典型代表污染物,开展OPFRs混合污染物微生物降解研究.结果表明:Bacillus pacificus能以4种OPFRs(TCEP、TCPP、TCP、TPhP)作为磷源生长代谢.在投菌量为1.0g/L,污染物初始浓度为1.0mg/L时,Bacillus pacificus对TCP的降解效果显著,在第9d的降解率达96.1%,另外3种OPFRs的降解率为23%~34%.4种OPFRs的主要降解途径均是通过磷酯键断裂,最终生成氯乙醇、氯丙醇、磷酸和苯类等小分子化合物.其中,TCEP和TCPP还可以通过末端脱氯进行分解,生成磷酸三丙酯和磷酸三(2-乙基丙基)酯,而TCP则还可以通过甲氧基化而被分解为磷酸三甲酯.研究表明:Bacillus pacificus能耐受并有效降解4种常见的OPFRs混合污染物,可为环境OPFRs污染治理提供新思路和优质的微生物菌种资源.In order to obtain more microbial species for degrading organophosphate flame retardants(OPFRs),and to reveal the pathway and mechanism of OPFRs biodegradation,the present isolated study a strain named Bacillus pacificus isolated from the residual sludge of a wastewater treatment plant in Guangzhou.The strain was proved to degrade a variety of OPFRs mixtures(including tris(2-chloroethyl)phosphate(TCEP),tris(2-chloropropyl)phosphate(TCPP),tritolyl Phosphate(TCP)and triphenyl phosphate(TPhP)).Bacillus pacificus used these four representative OPFRs as phosphorus sources for growth and metabolism.The results showed that 1.0mg/L TCP could be degraded efficiently by 1.0g/L of Bacillus pacificus,whose removal rate was 96.1%on the 9th day.While the biodegradation rates of other threes OPFRs were 23%to 34%.The four OPFRs were mainly degraded via phosphoester bond hydrolysis,and the final degradation produsts were small molecule compounds such as chloroethanol,chloropropanol,phosphoric acid,and benzene.In addition,TCEP and TCPP could be also decomposed by terminal dechlorination to produce tripropyl phosphate and tris(2-ethylpropyl)phosphate.TCP could be decomposed to trimethyl phosphate by methoxylation.The present study indicate that Bacillus pacificus can tolerate and effectively OPFRs mixtures,which can provide new ideas and high-quality microbial strain resources for OPFRs pollution management.

关 键 词：有机磷阻燃剂 微生物降解 混合污染 降解途径 太平洋芽孢杆菌 

分 类 号：X172[环境科学与工程—环境科学]