18β-甘草次酸对失血性休克大鼠肾损伤的保护作用  

Protective effect of 18β-glycyrrhetinic acid on the kidneys of rats with hemorrhagic shock

作　　者：盖倩 梁悦 王欣瑶 苏英 王慧慧 魏云昭 文佩 张娟娟 姜现瑞 GAI Qian;LIANG Yue;WANG Xin-Yao(Hexi University,Zhangye 734000,Gansu,China)

机构地区：[1]河西学院,甘肃张掖734000

出　　处：《吉林医学》2024年第6期1271-1275,共5页Jilin Medical Journal

基　　金：甘肃省高等学校创新基金项目[项目编号:2021A-119];河西学院校长基金青年科研项目[项目编号:QN2023006];国家级大学生创新训练计划项目[项目编号:202310740056];甘肃省大学生创新训练计划项目[项目编号:S202310740006]。

摘　　要：目的:探讨18β-甘草次酸(18β-Gly)对失血性休克(HS)大鼠急性肾损伤的保护作用。方法:将实验用SD雌性大鼠随机分为5组:假手术(SHAM)组、模型(HS)组、18β-Gly低剂量组、18β-Gly中剂量组、18β-Gly高剂量组,通过18β-Gly预处理,采用股动脉放血制作HS大鼠模型,检测大鼠HS后肾体比、肾组织病理形态改变,尿素氮(BUN)及血肌酐(CRE)的表达情况。结果:与HS组比较,18β-Gly中剂量组和18β-Gly高剂量组HS 2 h肾体比及CRE水平降低较明显,差异有统计学意义(P<0.05),而18β-Gly中剂量组BUN降低较明显,差异有统计学意义(P<0.05)。HE染色显示,与HS组比较,18β-Gly中剂量组和18β-Gly高剂量组病理损伤减轻较明显。结论:18β-Gly能有效降低HS大鼠肾/体比、BUN和CRE的水平,减轻HS大鼠肾组织病理损伤,起到肾保护作用。Objective To explore the protective effect of 18β-glycyrrhetinic acid 18β-Gly)on acute kidney injury in rats with hemorrhagic shock.Method SD female rats were randomly divided into 5 groups:SHAM group,HS group,18β-Gly low dose group,18β-Gly medium dose group and 18β-Gly high dose group,through 18β-Glycyrrhetinic acid pretreatment was used to create a hemorrhagic shock rat model using femoral artery bleeding.The renal body ratio,pathological changes in renal tissue,and expression of blood urea nitrogen(BUN)and creatinine(CRE)were detected after hemorrhagic shock in rats.Results Compared with the HS group,the renal body ratio and CRE levels in the 18β-Gly medium dose group and 18β-Gly high dose group were significantly decreased(P<0.05),but the BUN in the 18β-Gly medium dose group was significantly decreased(P<0.05).HE staining showed that the pathological damage was significantly reduced in the 18β-Gly medium dose group and 18β-Gly high dose group compared with the HS group.Conclusion 18β-Gly can effectively reduce the renal body ratio,BUN and CRE levels in hemorrhagic shock rats,reduce the renal pathological damage in hemorrhagic shock rats,and play a renal protective role.

关 键 词：18Β-甘草次酸 失血性休克 肾损伤 血肌酐 尿素氮 

分 类 号：R364.14[医药卫生—病理学]