燕麦SNP高密度遗传图谱构建及β-葡聚糖含量QTL定位  

Construction of SNP high-density genetic map and localization of QTL forβ-glucan content in oats

作　　者：韩丽[1] 汤胜胜 李佳 胡海斌 刘龙龙[1] 吴斌[2] HAN Li;TANG Sheng-Sheng;LI Jia;HU Hai-Bin;LIU Long-Long;WU Bin(Center for Agricultural Genetic,Resources Research,Shanxi Agricultural University/Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriclture and Rural Affairs,Taiyuan 030031,Shanxi,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]山西农业大学农业基因资源研究中心/农业农村部黄土高原作物基因资源与种质创新重点实验室,山西太原030031 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《作物学报》2024年第7期1710-1718,共9页Acta Agronomica Sinica

基　　金：财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-07-A);国家自然科学基金项目(30800699)资助。

摘　　要：β-葡聚糖是燕麦发挥保健作用的主要功能因子,提高其含量对优质燕麦生产有着重要意义。为促进高β-葡聚糖燕麦种质资源的有效利用和相关基因发掘,本研究以高β-葡聚糖品种夏莜麦和低β-葡聚糖品种赤38组配衍生的219个家系RIL8群体为材料,利用重测序技术构建了包含21个连锁群,5032个bin标记的遗传连锁图谱,图谱总长2045.09 cM,平均图距0.42 cM。利用标准酶法和近红外法对4个环境的RIL群体家系β-葡聚糖含量进行测定,结合测定结果,利用完备区间作图法对β-葡聚糖含量进行QTL定位分析,结果显示不同环境条件下RIL群体β-葡聚糖含量呈正态分布,并出现超亲后代家系,4个环境下群体β-葡聚糖含量变异系数介于9.06%~16.63%之间。QTL定位检测到7个与燕麦β-葡聚糖含量相关的QTL,分布于2D、3D、4C和4D染色体上,其中贡献率最高为14.73%,在2个环境中检测到同一个QTL,其标记区间为Chr4C_mark8361257–Chr4C_mark8384831。研究结果将为燕麦β-葡聚糖分子标记辅助育种提供重要的理论依据。β-glucan is the main functional component of oats for health care,and improving its content is of great significance to the production of high-quality oats.In this study,in order to promote the effective utilization of highβ-glucan oat germplasm resources and the discovery of related genes,a genetic linkage map containing 21 linkage groups and 5032 bin markers was constructed by using resequencing technology with the 223 RIL8 populations derived from the highβ-glucan variety Xiayoumai and the lowβ-glucan resource Chi38.The total length of the map was 2045.09 cM,and the average plot distance was 0.42 cM.Theβ-glucan content of RIL populations in four environments was determined by standard enzyme method and near infrared method.Combined with the determination results,QTL analysis ofβ-glucan content was performed by complete interval mapping method.The results showed that theβ-glucan content of RIL populations was normally distributed under different environmental conditions,and there were superparent descendants.The coefficient of variation ofβ-glucan content in the four environments ranged from 9.06%to 16.63%.Seven QTLs related toβ-glucan content in oat were detected by QTL mapping,distributed on 2D,3D,4C,and 4D chromosomes,with the highest contribution rate of 14.73%.The same QTL was detected in two environments with a marker interval of Chr4C_mark8361257–Chr4C_mark8384831.The results of this study provide an important theoretical basis for molecular marker-assisted breeding of oatβ-glucan.

关 键 词：燕麦 SNP 遗传图谱 Β-葡聚糖 QTL定位 

分 类 号：S512.6[农业科学—作物学]