蓝莓自花和异花授粉花柱转录组SSR位点分析  

Analysis of SSR loci in blueberry self-pollinated and cross-pollinated styles transcriptome

作　　者：杨芩 付燕[3] 邓洁[1,2] 张婷渟 刘雅兰[1,2] YANG Qin;FU Yan;DENG Jie;ZHANG Tingting;LIU Yalan(Key Laboratory of Molecular Breeding and Variety Creation of Horticultural Plants for Mountain Features in Guizhou Province,Kaili 556000,China;Development Planning Division,Kaili University,Kaili 556000,China;Qiandongnan Vocational and Technical College,Kaili 556000,China)

机构地区：[1]贵州山地特色园艺作物分子育种与品种创制重点实验室,贵州凯里556000 [2]凯里学院发展规划处,贵州凯里556000 [3]黔东南民族职业技术学院,贵州凯里556000

出　　处：《安徽农学通报》2024年第11期33-41,共9页Anhui Agricultural Science Bulletin

基　　金：贵州省“千”层次创新型人才培养项目(黔千层人才〔2021〕201402);凯里学院农业硕士专业学位培育点专项课题(kysszx2022014);凯里学院贵州省省级一流本科专业(园艺);凯里学院一流学科(园艺学)。

摘　　要：为鉴定蓝莓自交不亲和基因,本研究对“杰兔”蓝莓品种自花和异花授粉花柱进行了转录组测序,对简单重复序列(SSR)位点进行分析,对含SSR位点且差异表达的非冗余序列(Unigene)进行基因本体论(GO)功能富集和京都基因与基因组数据库(KEGG)注释分析,并开展SSR标见引物设计。结果表明,蓝莓花柱转录组共获得85890条Unigene,其中有21962个Unigene含有26557个SSR位点,发生频率为25.57%。在所有SSR位点中包含有297种重复基元,以单核苷酸和二核苷酸重复为主要重复类型,占总SSR的89.52%;其中A/T类型和AG/CT类型SSR位点数占SSR总数的83.44%。针对所有SSR位点,共设计出17212对引物,成功率78.37%。差异转录组筛选到58、27、16、3、2和1条含有SSR位点的差异表达Unigene,分别与植物激素信号转导、细胞骨架、蛋白质泛素化、花粉管生长、核糖核酸酶T2家族和细胞程序化死亡等代谢途径相关。本研究丰富了蓝莓的EST-SSR分子标记,为蓝莓自交不亲和的分子机制研究提供了参考。In order to carry out the identification of self-incompatibility genes in blueberry,transcriptome se⁃quencing was performed on the self-pollinated and cross-pollinated styles of blueberry cultivar“Premier”in this study,and simple repeat sequence(SSR)loci were analyzed.On this basis,SSR loci of assembled and redundant Unigene were analyzed,as well as it was conducted that the significant enrichment analysis of GO and KEGG for the differential ex⁃pression of unigenes containing SSR,and the SSR primers were designed.The results showed that a total of 85890 unigenes were obtained from the blueberry styles transcriptome,of that 21962 Unigene were found to contain 26557 SSR loci,with a frequency of 25.57%.The mononucleotide and dinucleotide repeats were the main repeat types of the 297 kinds of repeat motif in all SSR loci,accounting for 89.52%of the total SSR,of which the number of A/T and AG/CT SSR locations accounted for 83.44%of the total number of SSR locations.A total of 17212 pairs of primers were de⁃signed for all SSR loci,with a success rate of 78.37%.The differential transcripome combined with SSR loci information showed that 58,27,16,3,2 and 1 unigene with SSR sites were screened,which were related to plant hormone signal transduction,cytoskeleton,protein ubiquitination,pollen tube growth,ribonucnase T2 family,and programmed cell death,respectively.This study not only enriched EST-SSR markers of blueberry,but also laid a foundation for molecu⁃lar mechanism of self-incompatibility in blueberry.

关 键 词：蓝莓 自花授粉 异花授粉 花柱 转录组 简单重复序列 

分 类 号：S603.2[农业科学—园艺学]