人工诱导东北林蛙苏醒适应机制分析  

Analysis on the mechanism of artificial induction of awakening adaptation in Rana dybowskii

作　　者：赵红蕾 张传辉[1] 孟如愿 马佳欣 文仁霜 于翠平[1] ZHAO Honglei;ZHANG Chuanhui;MENG Ruyuan;MA Jiaxin;WEN Renshuang;YU Cuiping(College of Wildlife and Protected Area Northeast Forestry University,Harbin 150040,China)

机构地区：[1]东北林业大学野生动物与自然保护地学院,黑龙江哈尔滨150040

出　　处：《安徽农学通报》2024年第11期49-55,共7页Anhui Agricultural Science Bulletin

基　　金：中央高校基本科研业务费专项资金项目(2572022BE01)。

摘　　要：为了探究东北林蛙在人工诱导苏醒过程中适应温度变化的生理机制,包括不同温度、不同器官或组织的冷冻保护剂含量变化及其抗氧化应激相关生理指标在苏醒适应阶段的差异,采用梯度升温方法对冬眠东北林蛙进行人工诱导苏醒,至东北林蛙摄食标记苏醒过程结束。苏醒培养期间取样,未取样林蛙持续培养,取样结束后剩余林蛙常温养殖;采用分光光度法对其肝脏、肌肉内的葡萄糖含量、尿素氮含量、总抗氧化能力进行测定。结果表明,东北林蛙肝脏内上述物质含量与肌肉内各物质的消耗趋势基本一致,但肝脏内各物质含量及其变化幅度远大于肌肉内。冬眠东北林蛙人工诱导苏醒初期葡萄糖、尿素氮含量即冷冻保护剂含量明显降低(P<0.05)、总抗氧化能力明显增强(P<0.05),而苏醒后期,各项含量趋于稳定,无明显变化。这说明肝脏是东北林蛙冬眠过程中对抗低温冷冻环境的主要器官,且冬眠状态的东北林蛙体内会积累冷冻保护剂,随着苏醒过程的进行,温度逐渐上升,机体活动逐渐恢复,葡萄糖被大量消耗,以对抗因缺少能量来源而带来的应激损伤,抗氧化应激能力也随之增强以维持内环境稳定,保证东北林蛙在0℃以下得以生存。In order to explore the physiological mechanisms by which the Rana dybowskii adapts to temperature changes during artificial induction of awakening,including changes in cryoprotectant content at different temperatures and organs/tissues,as well as differences in antioxidant stress-related physiological indicators during the awakening adaptation stage,a gradient heating method was used to artificially induce awakening of the hibernating R.dybowskii,until the awakening process was marked by feeding by the R.dybowskii.During the awakening cultivation period,samples were taken,and non-sampled forest frogs were continuously cultured.After the sampling was completed,the remaining forest frogs were raised at room temperature;and the glucose content,urea nitrogen content,and total antioxidant capacity in its liver and muscles were measured using spectrophotometry.The results showed that the content of various substances in the liver was basically consistent with the trend of muscle consumption,but the content and changes of various substances in the liver were much greater than those in the muscle.The glucose and urea nitrogen content(cryoprotectant content)were significantly reduced(P<0.05),and the total antioxidant capacity was significantly enhanced(P<0.05)in the early stage of artificial induction and awakening of the hibernating R.dybowskii.However,in the later stage of awakening,the various contents tended to stabilize without significant changes.The liver is the main organ that fights against the low-temperature freezing environment during the hibernation process of the R.dybowskii,and the hibernating R.dybowskii accumulates cryoprotectants in its body.As the awakening process progresses,the temperature gradually rises,and the body activity gradually recovers.The glucose is consumed in large quantities,reducing stress damage caused by a lack of energy sources,and the antioxidant stress capacity is also enhanced to maintain internal environment stability,ensure the survival of R.dybowskii below 0℃celsius.

关 键 词：东北林蛙 人工诱导苏醒 冷冻保护剂 抗氧化能力 人工养殖 

分 类 号：S966.35[农业科学—水产养殖]