健康和物理损伤条件下椰树叶柄挥发物组分比较分析  

Comparative analysis of volatile components in Cocos nucifera leaf under healthy and physically damaged conditions

作　　者：刘博 阎伟[1] 刘丽[1] LIU Bo;YAN Wei;LIU Li(Coconut Research Institute,Chinese Academy of Tropical Agricultural Sciences,Wenchang 571339,China)

机构地区：[1]中国热带农业科学院椰子研究所,海南文昌571339

出　　处：《安徽农学通报》2024年第11期59-63,共5页Anhui Agricultural Science Bulletin

基　　金：海南省自然科学基金(321QN344);海南省重点研发计划项目(ZDYF2020083)。

摘　　要：椰树叶柄挥发物对锈色棕榈象成虫具有吸引作用探索不同状态下叶柄挥发物组分可分析该昆虫的定向行为。为了明确健康和物理损伤椰树叶柄释放的挥发物组分,本文采用顶空采集法收集健康和物理损伤后椰树叶柄挥发物,利用气相色谱-质谱联用技术(GC-MS)检测其挥发性化合物成分及相对含量,并对健康和物理损伤椰树叶柄挥发物组分进行了比较分析。结果表明,健康叶柄共检测出挥发性物质53种,包括烃类26种、腈类2种、酮类5种、酯类5种、茚类6种、醇类5种和其他物质4种,其中相对含量较高的5个成分为3a,4,4a,5,8,8a,9,9a-八氢-4,9:5,8-二亚甲基-1H-苯并[f]茚、丁基化羟基甲苯、L-α-松油醇、哒嗪和双环戊二烯;物理损伤叶柄共检测出挥发性物质51种,包括烃类25种、腈类5种、酮类5种、酯类6种、茚类5种、醇类3种和其他物质2种,其中相对含量较高的5个成分为3a,4,4a,5,8,8a,9,9a-八氢-4,9:5,8-二亚甲基-1H-苯并[f]茚、丁基化羟基甲苯、双环[2.2.2]辛-5-烯-2-甲腈、α-松油醇和2,6-二甲基-4-氧杂三环[5.2.1.0(2,6)]癸-8-烯-3-酮。健康与物理损伤叶柄挥发物主要组分大体一致,在具体种类和含量上存在一定差异。研究结果可为揭示锈色棕榈象成虫寄主定位行为的化学通信机制及研制植物源引诱剂提供参考。Cocos nucifera leaf petiole volatiles exhibit an attractive effect on the Rhynchophorus ferrugineu,and exploring the volatile components of petioles under different states could analyzed the directional behavior of this insect.In order to elucidate the volatile components released from healthy and physically damaged Cocos nucifera leaf petioles,this study employed headspace sampling to collect the volatiles.Gas chromatography-mass spectrometry(GC-MS)was utilized to analyze the composition and relative abundance of volatile compounds,and a comparative analysis of the volatile components of healthy and physically damaged Cocos nucifera leaf petioles was conducted.The results indicated that a total of 53 volatile substances were detected in healthy petioles,including 26 hydrocarbons,2 nitriles,5 ketones,5 esters,6 indenes,5 alcohols and 4 other compounds.Among them,the 5 components with higher relative abundances were 4,9:5,8-dimethano-1H-benz[f]indene,3a,4,4a,5,8,8a,9,9a-octahydro-,Butylated Hydroxytoluene,L-.alpha.-Terpineol,Pyridazine,and Dicyclopentadiene.Physically damaged petioles emitted a total of 51 volatile substances,comprising 25 hydrocarbons,5 nitriles,5 ketones,6 esters,5 indenes,3 alcohols and 2 other compounds.Among them,the 5 components with higher relative abundances were 4,9:5,8-dimethano-1H-benz[f]indene,3a,4,4a,5,8,8a,9,9aoctahydro-,Butylated Hydroxytoluene,Bicyclo[2.2.2]oct-5-ene-2-carbonitrile,.alpha.-Terpineol,and 2,6-Dimethyl-4-oxatricyclo[5.2.1.0(2,6)]dec-8-en-3-one.The volatiles emitted from physically damaged petioles were generally similar to those from healthy petioles in terms of major components,but there were still some differences in specific types and relative abundances.The research results could provide a reference for elucidating the chemical communication mechanisms underlying the host-location behavior of the Rhynchophorus ferrugineus and for the development of plant-based attractants.

关 键 词：椰子树 气相色谱-质谱联用技术 植物挥发物 叶柄 物理损伤 

分 类 号：S31[农业科学—作物栽培与耕作技术]