宜宾4个品种早茶叶可培养内生细菌的比较分析  

Comparative Analysis of Culturable Endophytic Bacteria Communities in Leaves of 4 Early Tea Plant Varieties Planted in Yibin City

作　　者：邓骛远[1] 高刚[1] 游玲[1] 张海英[1] DENG Wuyuan;GAO Gang;YOU Ling;ZHANG Haiying(Faculty of Agriculture,Forestry and Food Engineering of Yibin University,Yibin,Sichuan 6444000,China)

机构地区：[1]宜宾学院农林与食品工程学部,四川宜宾644000

出　　处：《宜宾学院学报》2024年第6期59-64,共6页Journal of Yibin University

基　　金：四川省科技厅面上项目(2021YJ0292);固态发酵资源利用四川省重点实验室开放基金(2018GTJ002);香料植物资源开发与利用四川省高校重点实验室开放基金(2018XLZ006)。

摘　　要：为了比较宜宾不同品种早茶叶可培养内生细菌群落结构,以种植面积较广的4个早茶品种为材料,采用传统培养方法,分别从其老叶和嫩叶中分离内生细菌,并经16S rDNA序列分析对其进行鉴定.结果表明,从4个品种早茶叶中共分离到内生细菌336株,归类于18个属23个种.从内生细菌数量和多样性来看,黄芽早>福鼎大白>中茶302>中茶108,且均为嫩叶>老叶.微杆菌属Microbacterium和黄单胞菌属Xanthomonas在4个品种早茶叶中均有分布,且都集中或仅存在于嫩叶,其中Microbacterium为绝对优势菌群.各种早茶老叶和嫩叶还有各自的优势菌属短小杆菌属Curtobacterium等或特异性菌种短小芽胞杆菌B. pumilus、磷酸镰孢菌F. phosphorivorans等.说明不同品种早茶叶内生细菌种类组成有明显的相似性,也存在一定的品种或组织专一性.这些内生细菌及其分布规律可能在较大程度上促成了宜宾早茶的高产优质以及各品种早茶的不同品质特征.In order to compare and analyze the culturable endophytic bacteria communities in leaves of different early tea plant varieties,t he endophytic bacteria from the leaves of 4 representative early tea plant varieties planted in Yibin were isolated,and all these strains were identified by the 16S rDNA analysis.The results show that a total of 336 strains of endophytic bacteria are ob⁃tained in 4 early tea leaves,which are classified into 18 genera and 23 species.The number and diversity of endophytic bacteria vary among tea varieties,Huangyazao>Fudingdabai>Zhongcha 302>Zhongcha 108,and young leaves>old leaves.Microbac⁃terium and Xanthomonas are found in all early tea plant varieties,and they concentrate or only exist in young leaves of various vari⁃eties,and Microbacterium is the dominant genus.The old and(or)young tea leaves also have their own dominant bacteria genus Curtobacterium or specific strains B.pumilus,F.phosphorivorans and so on.There are obvious similarities in the composition of endo⁃phytic bacteria in different varieties of early tea plant,but there is also a variety specificity or tissue specificity.These endophytic bacteria and their distribution in leaves of early tea plant in Yibin may contribute to the high tea yield and quality,and the differ⁃ent quality characteristics of various varieties.

关 键 词：宜宾 早茶 可培养内生细菌 

分 类 号：Q939.11[生物学—微生物学]