MABR好氧喹啉和苯酚降解驱动反硝化脱氮研究  

Aerobic Biodegradation of Quinoline and Phenol Combined with Denitrification in A Membrane-Aerated Biofilm Reactor

作　　者：惠明[1,2] 郑桂朋 胡腾宇 李园园 田海龙 李保安[4] HUI Ming;ZHENG Guipeng;HU Tengyu;LI Yuanyuan;TIAN Hailong;LI Baoan(College of Biological Engineering,Henan University of Technology,Zhengzhou 450001,China;Henan Provincial Engineering Laboratory of Preservation and Breeding of Industrial Microbial Strains,Zhengzhou 450001,China;Agricultural College,Henan University,Kaifeng 475001,China;College of Environment Science and Engineering,Nankai University,Tianjin 300071,China)

机构地区：[1]河南工业大学生物工程学院,河南郑州450001 [2]工业微生物菌种选育与保藏河南省工程实验室,河南郑州450001 [3]河南大学农学院,河南开封475001 [4]南开大学环境科学与工程学院,天津300071

出　　处：《水处理技术》2024年第6期52-56,68,共6页Technology of Water Treatment

基　　金：河南省科技攻关项目(222102320138);河南省科技研发计划联合基金(应用攻关类)项目(232103810068)。

摘　　要：以喹啉和苯酚为含氮杂环类和酚类污染物为代表的废水是典型的强毒性难降解有机废水。该课题采用无泡膜曝气生物膜反应器(MABR)实现了好氧反硝化驱动喹啉和苯酚降解生物强化过程。反应器在不同工况条件下,完成了对喹啉、苯酚和硝酸盐分别为(82.6±10.2)%、(84.5±11.9)%、(67.3±10.6)%的去除率以及(11.1±1.4)、(3.1±1.2)、(1.91±0.4)g/(m^(2)·d)的去除负荷。胞外聚合物含量的增加有助于提高生物膜对高喹啉进水负荷的抵抗能力。通过解析生物膜菌群结构,发现生物膜能够有效富集Rhodococcus等典型好氧喹啉降解菌;通过q-PCR功能基因定量分析发现以oxoO为代表的喹啉降解基因和napA好氧反硝化指示基因存在同一菌群,说明了好氧反硝化驱动喹啉和苯酚降解菌群的存在。通过宏基因组学分析,进一步核定Pseudomonas和Raineyella是主要的好氧喹啉和苯酚降解协同反硝化功能的关键菌群。本课题证实了MABR生物膜在好氧条件下完成对喹啉和苯酚的高效降解和好氧反硝化耦合过程,为拓展MABR在含氮杂环和酚类有机废水处理的应用提供必要的理论参考。Quinoline and phenol,the representatives of nitrogenous heterocyclic compounds and phenolic compounds,are difficult to degrade in industrial organic wastewater.This research applied a membrane-aerated biofilm reactor(MABR)to achieve the aerobic degradation of quinoline and phenol driving the denitrification process.The reactor exhibited efficient removal performance of(82.6±10.2)%,(84.5±11.9%)and(67.3±10.6)%for quinoline,phenol and nitrate under different operational conditions,as well as the removal rates of(11.1±1.4,)(3.1±1.2)and(1.91±0.4)g/(m^(2)·d,)respectively.The increased extracellular polymeric substances(EPS)contributed to enhancing the resistance of biofilms against high quinoline and phenol shocks.By analyzing the microbial community structure of biofilms,it was found that the biofilms could effectively enrich aerobic quinoline-degrading bacteria such as Rhodococcus.The co-occurrence of aerobic quinoline degradation gene oxoO and denitrifying gene napA in the same microbial populations indicated the existence of aerobic denitrification driven by quinoline and phenol degradation.Metagenomic analysis further confirmed that Pseudomonas and Raineyella were the key microbial groups responsible for the synergistic functions of aerobic quinoline and phenol degradation combined with denitrification.The results advance our understanding of quinoline and phenol degradation during biological nitrogen removal,and highlight the potential implementation of aerobic denitrification-driven nitrogenous heterocyclic compounds and phenolic compounds from organic industrial wastewaters in MABR.

关 键 词：生物膜 好氧反硝化 喹啉 苯酚 生物降解 

分 类 号：X172[环境科学与工程—环境科学] X703