糙皮侧耳原基发育关联物质及代谢通路分析  

Analysis of associated substances and metabolic pathways involved in primordium development of Pleurotus ostreatus

作　　者：刘芹[1] 孔维丽[1] 崔筱[1] 牛森园 师子文 吴杰 梁雪迪 刘阳 LIU Qin;KONG Weili;CUI Xiao;NIU Senyuan;SHI Ziwen;WU Jie;LIANG Xuedi;LIU Yang(Institute of Edible Fungi,Henan Academy of Agricultural Sciences/Key Laboratory of Evaluation and Utilization of Edible Fungi Germplasm Resources in Huang-Huai-Hai Region,Ministry of Agriculture and Rural Affairs,Zhengzhou 450002,Henan,China;College of Life Science and Technology,Henan Institute of Science and Technology,Xinxiang 453003,Henan,China;College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,Henan,China)

机构地区：[1]河南省农业科学院食用菌研究所·农业农村部黄淮海地区食用菌种质资源鉴定和利用重点实验室,郑州450002 [2]河南科技学院生命科学学院,河南新乡453003 [3]河南农业大学生命科学学院,郑州450002

出　　处：《中国瓜菜》2024年第6期52-60,共9页China Cucurbits And Vegetables

基　　金：河南省现代农业产业技术体系(HARS-22-08-S,HARS-22-08G1)。

摘　　要：为揭示糙皮侧耳原基发育的潜在调控物质,采用代谢组学的方法对糙皮侧耳菌丝体和原基细胞代谢物进行检测分析。结果表明,菌丝体与原基细胞的代谢物具有显著差异。采用正交偏最小二乘判别分析(OPLS‐DA),以VIP(varible importance in the projection)≥1和倍数变化≥2或≤0.5为条件共筛选到400种差异代谢物。调控通路分析表明,这些差异物质涉及33条代谢通路,其中丙氨酸、天冬氨酸和谷氨酸、氨酰tRNA生物合成、嘌呤代谢等8条代谢通路具有极显著影响。谷氨酸和谷氨酰胺涉及p<0.01的大部分代谢通路,可能在糙皮侧耳原基发育过程中具有重要调控作用。研究结果为糙皮侧耳以及其他大型真菌原基发育机制的探索提供了理论依据。In order to reveal the potential regulatory substances involved in primordium development of Pleurotus ostreatus,the metabolomics was used to detected the cellular metabolites in mycelium and primordium.The results showed that there were significant differences between the cellular metabolites in mycelium and primordium.A total of 400 differential metabolites were screened under the condition of VIP(varible importance in the projection)≥1 and fold change≥2 or≤0.5 using orthogonal partial least squares discriminant analysis(OPLS-DA).According to pathway analysis,these differential substances might involve 33 metabolic pathways,among which 8 metabolic pathways,including alanine,aspartate and glutamate,aminoacyl tRNA biosynthesis and purine metabolism,had extremely significant effects.Glutamate and glutamine involved in most of the metabolic pathways with p<0.01,therefore they might play an important regulatory role in primordium development of P.ostreatus.This study provides a theoretical basis for exploring the development mechanism of P.ostreatus and other macrofungus.

关 键 词：糙皮侧耳 原基发育 代谢组学 差异代谢物 代谢通路 

分 类 号：S646.141[农业科学—蔬菜学]