不同时间急性睡眠剥夺对大鼠行为学及突触生物标志物表达的影响  

Effects of acute sleep deprivation on behavior and synaptic biomarker expression in rats

作　　者：张士滨 王璐[1] 王储 郭鹏程 闫旭升[2] 霍东升[2] 杨占君[2] 王艳国[2] 贾建新[2] ZHANG Shibin;WANG Lu;WANG Chu;GUO Pengcheng;YAN Xusheng;HUO Dongsheng;YANG Zhanjun;WANG Yanguo;JIA Jianxin(Graduate School of Baotou Medical College,Inner Mongolia University of Science and Technology,Baotou 014040,China;Department of Human Anatomy,Baotou Medical College,Inner Mongolia University of Science and Technology,Baotou 014040)

机构地区：[1]内蒙古科技大学包头医学院研究生学院,内蒙古包头014040 [2]内蒙古科技大学包头医学院人体解剖学教研室,内蒙古包头014040

出　　处：《中国比较医学杂志》2024年第5期55-64,共10页Chinese Journal of Comparative Medicine

基　　金：内蒙古自治区高等学校“青年科技英才支持计划”(NJYT-20-A08);内蒙古自治区高等学校创新团队发展计划(NMGIRT2328);内蒙古自然科学基金(2021LHMS08018);国家自然科学基金(81860215)。

摘　　要：目的探究不同时间急性睡眠剥夺对大鼠行为学及突触蛋白表达的影响。方法健康雄性Wistar大鼠70只随机分为7组,即空白对照组(Control)、睡眠剥夺24 h组、48 h组、72 h组、96 h组、120 h组、144 h组,采用改良多平台水环境睡眠剥夺法建立大鼠睡眠剥夺模型,通过Morris水迷宫检测空间学习记忆能力,旷场实验检测大鼠焦虑状况,Nissl染色观察大鼠海马神经元形态、数量的改变,Western blot、Real-time PCR实验分别测定大鼠突触相关分子标志物突触素(synaptophysin,SYN)、突触后膜致密物质95(post-synaptic density protein-95,PSD-95)、脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)的表达。结果与Control组比较,随着睡眠剥夺时间的延长,大鼠的站立次数和修饰行为次数均显著增加(P<0.05);120 h、144 h组大鼠的逃避潜伏期与上台前路程均显著增加(P<0.05),而穿越平台次数和目标象限时间百分比均显著下降(P<0.01),且与睡眠剥夺时间呈负相关;BDNF、SYN及PSD-95蛋白/基因的表达量均随睡眠剥夺时间的延长呈明显下降趋势(P<0.01),且与睡眠剥夺时间呈负相关。结论随着睡眠剥夺时间的增加,模型大鼠空间学习记忆损害与焦虑情绪逐渐加重,其可能与突触相关分子标志物表达减少有关。Objective To investigate the effects of acute sleep deprivation on the behavior and synaptic protein expression of rats.Methods Seventy healthy male Wistar rats were randomly divided into seven groups,a Control group and sleep deprivation groups(24,48,72,96,120 and 144 hours).The sleep deprivation rat model was established by the modified multiplatform water environment sleep deprivation method.Spatial learning and memory were assessed by the Morris water maze.Anxiety was assessed by the open field test.The morphology and quantity of hippocampal neurons were observed by Nissl staining.Western blot and Real-time PCR were used to determine the expression of synaptophysin(SYN),post-synaptic density protein-95(PSD-95),and brain-derived neurotrophic factor(BDNF)in rats.Results Compared with the Control group,the numbers of standing and modification were significantly increased by prolongation of the sleep deprivation time(P<0.05).The escape latency and path length were significantly increased in 120 and 144 h groups(P<0.05),whereas the number of platform crossings and the percentage of the target quadrant time were significantly decreased(P<0.01)and negatively correlated to the sleep deprivation time.The expression levels of BDNF,SYN,and PSD-95 were significantly decreased with the prolongation of sleep deprivation time(P<0.01).Conclusions With the increase in sleep deprivation time,cognitive dysfunction and anxiety gradually deteriorated,which may be related to decreases in the expression of synaptic biomarkers.

关 键 词：大鼠 睡眠剥夺 学习认知 海马 突触可塑性 

分 类 号：R-33[医药卫生]