氨氮胁迫对两种泥鳅急性毒性及抗氧化功能的影响  

Effects of ammonia nitrogen stress on acute toxicity and antioxidant function of two species of mud fish

作　　者：李建平 胡强 夏英 张桂元 湛栋 李永吉 LI Jianping;HU Qiang;XIA Ying;ZHANG Guiyuan;ZHAN Dong;LI Yongji(Yueyang Academy of Agricultural Science,Yueyang 414000,China;Yueyang Aquatic Germplasm Resources Protection Center,Yueyang 414020,China)

机构地区：[1]岳阳市农业科学研究院,湖南岳阳414000 [2]岳阳市水产种质资源保护中心,湖南岳阳414020

出　　处：《渔业研究》2024年第3期254-259,共6页Journal of Fisheries Research

基　　金：湖南省水产产业技术体系项目(湘农发﹝2019﹞105号)。

摘　　要：为研究氨氮胁迫对泥鳅(Misgurnus anguillicaudatus)和大鳞副泥鳅(Paracheilus dabryanus)急性毒性及抗氧化功能的影响,采用静态毒性试验方法,以平均体质量为(8.13±1.04)g的泥鳅和平均体质量为(8.72±1.33)g的大鳞副泥鳅为试验对象,得到氨氮对泥鳅和大鳞副泥鳅72 h半致死浓度分别为610.45、516.90 mg/L,对应的非离子氨(NH_(3))浓度分别为5.04和4.26 mg/L。基于半致死浓度,研究了100 mg/L氨氮暴露对两种泥鳅肝脏和肌肉组织的抗氧化功能的影响,发现氨氮胁迫后MDA含量,以及SOD、LZM和GPx活性均出现不同程度的升高,且泥鳅的抗氧化酶活性略高于大鳞副泥鳅。试验结果表明,泥鳅的抗氧化功能强于大鳞副泥鳅。To investigate the impact of ammonia nitrogen stress on the acute toxicity and antioxidant responses of Misgurnus anguillicaudatus and Paracheilus dabryanus,a static toxicity test was conducted on M.anguilli-caudatus[body weight(8.13±1.04)g]and P.dabryanus[body weight(8.72±1.33)g].The semi-lethal con-centrations of ammonia nitrogen over 72 hours were determined as 610.45 mg/L and 516.90 mg/L for M.an-guillicaudatus and P.dabryanus,respectively.Correspondingly,the non-ionic ammonia concentrations were found to be 5.04 mg/L and 4.26 mg/L.Upon exposure to a semi-lethal mass concentration of 100 mg/L ammo-nia nitrogen,the impact on the antioxidant function of the liver and muscles in both mud fish species was ex-amined.The results indicated that following ammonia nitrogen stress,the levels of malondialdehyde(MDA),superoxide dismutase(SOD),lysozyme(LZM),and glutathione peroxidase(GPx)activities all exhibited vary-ing degrees of increase.Notably,the antioxidant enzyme activity of M.anguillicaudatus surpassed that of P.dabryanus.These findings suggest that M.anguillicaudatus displays a more robust antioxidant response com-pared to P.dabryanus under ammonia nitrogen stress conditions.

关 键 词：泥鳅 大鳞副泥鳅 氨氮胁迫 半致死浓度 抗氧化功能 

分 类 号：S948[农业科学—水产养殖]