藻岩黄质对人宫颈癌HeLa细胞增殖和凋亡的影响  

Effect of fucoxanthin on viability and apoptosis of human cervical cancer HeLa cells

作　　者：叶国柳 王玲玲 杨康 常梦然 YE Guoliu;WANG Lingling;YANG Kang;CHANG Mengran(Department of Obstetrics and Gynecology,The First Affiliated Hospital of Bengbu Medical University,Bengbu,Anhui 233004,China)

机构地区：[1]蚌埠医科大学第一附属医院妇科,安徽蚌埠233004

出　　处：《淮海医药》2024年第3期236-239,244,共5页Journal of Huaihai Medicine

基　　金：安徽省高等学校自然科学重点研究项目(2022AH051428);安徽省教育厅2021高校拔尖人才培育资助项目(gxyq2021190)。

摘　　要：目的:探讨藻岩黄质对宫颈癌HeLa细胞增殖和凋亡的影响,分析其抑制肿瘤生长的机制。方法:体外培养HeLa细胞,设为给药组和空白对照组,空白对照组采用常规细胞培养,给药组加入一定浓度的藻岩黄质培养。采用MTT法检测不同浓度(0.1、0.5、1μmol/L)藻岩黄质处理HeLa细胞24 h及0.5μmol/L藻岩黄质处理HeLa细胞12、24、48 h后,HeLa细胞存活率的变化;采用Annexin V/PI流式细胞仪分别检测0.1μmol/L、0.5μmol/L藻岩黄质刺激HeLa细胞24 h及48 h后的凋亡情况;测定0.1、0.5、1μmol/L的藻岩黄质刺激HeLa细胞后培养基中ATP的生成和总氧摄取量。结果:与空白对照组相比,随着药物浓度增加,HeLa细胞生长逐渐缓慢(P<0.05);随着药物浓度和刺激时间增加,HeLa细胞凋亡率显著增加(P<0.05);随着药物刺激浓度增加,HeLa细胞ATP的产生率也显著下降(P<0.05)。结论:藻岩黄质能够抑制宫颈癌HeLa细胞活力并诱导其凋亡。Objective:To investigate the effect of fucoxanthin on proliferation and apoptosis of HeLa cells and to analyze its mechanism of inhibiting tumor growth.Methods:A comparison was conducted between a treatment group of cervical cancer HeLa cells with fucoxanthin and a blank control group.MTT method was used to detect HeLa cells treated with different concentrations of fucoxanthin for 24 hours and the changes in the survival rate of HeLa cells after 12 hours,24 hours,and 48 hours of treatment with 0.5 umol/L of fucoxanthin were observed.The apoptosis of HeLa cells was detected using Annexin V/PI flow cytometry at 24 hours and 48 hours after stimulation with 0.1umol/L and 0.5umol/L of fucoxanthin,respectively.The production of ATP and total oxygen uptake were determined after the culture medium of HeLa cells were stimulated with 0.1 umol/L,0.5 umol/L,and 1 umol/L of fucoxanthin,respectively.Results:Compared with the blank controls,as the concentration increased,the inhibitory effect of fucoxanthin on HeLa cell growth gradually increased(P<0.05).At the same time,as the dosage and stimulation time of fucoxanthin increased,the apoptosis rate of HeLa cells significantly increased(P<0.05).As the concentration increased,the ATP production rate of HeLa cells stimulated by fucoxanthin also significantly decreased(P<0.05).Conclusion:Fucoxanthin can inhibit the growth and induce apoptosis of cervical cancer HeLa cells.

关 键 词：宫颈肿瘤 藻岩黄质 HELA细胞 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]