亮蓝G通过抑制P2X7/NLRP3通路减轻脑缺血再灌注大鼠神经炎症  

Brilliant blue G alleviates neuroinflammation in cerebral ischemia-reperfusion rats by inhibiting the P2X7/NLRP3 pathway

作　　者：王振 张冲 石佐林 董玉书 WANG Zhen;ZHANG Chong;SHI Zuolin;DONG Yushu(Department of Neurosurgery,General Hospital of Northern Theater Command of Chinese People’s Liberation Army,Shenyang 110016,China)

机构地区：[1]中国人民解放军北部战区总医院神经外科,沈阳110016

出　　处：《神经解剖学杂志》2024年第2期219-223,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(82071481)。

摘　　要：目的:研究嘌呤受体P2X7在脑缺血再灌注损伤(CIRI)中的作用。方法:使用右侧大脑中动脉闭塞法(MCAO)制备大鼠CIRI模型,通过腹腔注射给予P2X7抑制剂亮蓝G(BBG)或NLRP3抑制剂MCC950处理。神经功能评分检测大鼠神经功能的改变,伊文思蓝染色检测血脑屏障完整性,通过酶联免疫吸附试验(ELISA)检测大鼠脑脊液中IL-1β和IL-18的含量,通过Western Blot检测右侧大脑皮质中P2X7、CD11b和NLRP3的表达。结果:BBG或者MCC950处理能改善CIRI大鼠神经功能,同时降低脑组织中伊文思蓝的含量,并降低脑脊液中IL-1β和IL-18的水平。此外,BBG可以降低右侧大脑皮质中CD11b和NLRP3的表达,但是MCC950只能降低CD11b表达,对P2X7表达没有明显影响。结论:BBG通过抑制P2X7/NLRP3通路减轻CIRI大鼠神经炎症。Objective:To investigate the role of purine receptor P2X7 in cerebral ischemia-reperfusion injury(CIRI).Methods:The CIRI model was prepared by right middle cerebral artery occlusion(MCAO).The P2X7 inhibitor bright blue G(BBG)or NLRP3 inhibitor MCC950 were injected intraperitoneally.Neurological function score was used to detect the changes of neural function in rats.The integrity of blood-brain barrier was detected by Evans blue staining.The contents of IL-1βand IL-18 in cerebrospinal fluid(CSF)of rats were detected by ELISA,and the expressions of P2X7,CD11b,and NLRP3 in the right cerebral cortex were detected by Western Blot.Results:Treatment with BBG or MCC950 improved neural function in CIRI rats,while reducing the amount of Evans blue in brain tissue and IL-1βand IL-18 levels in CSF.In addition,BBG can reduce the expression of CD11b and NLRP3 in the right cerebral cortex,while MCC950 can only reduce the expression of CD11b,but has no effect on P2X7 expression.Conclusion:BBG alleviates neuroinflammation in CIRI rats by inhibiting P2X7/NLRP3 pathway.

关 键 词：脑缺血再灌注损伤 P2X7/NLRP3通路 亮蓝G 小胶质细胞 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]