柴术理胃饮对肝郁脾虚型功能性消化不良大鼠胃肠动力及p-NF-κBp65通路的影响  

Effect of Chaizhu Liwei Decoction on Gastrointestinal Motility and NF-κBp65 Pathway in Functional Dyspepsia Rats with Liver Depression and Spleen Deficiency

作　　者：范明明 张艮霜 常雨 刘永梅 张艺川 李显筑 FAN Mingming;ZHANG Genshuang;CHANG Yu(Heilongjiang College of Traditional Chinese Medicine,Heilongjiang 150000,China)

机构地区：[1]黑龙江省中医药科学院南岗院区老年病科,哈尔滨150000 [2]黑龙江省中医药科学院南岗院区内科,哈尔滨150000

出　　处：《医学研究杂志》2024年第5期74-79,共6页Journal of Medical Research

基　　金：黑龙江省自然科学基金资助项目(LH2023H079);黑龙江省博士后基金资助项目(040102-438);黑龙江省卫生健康委员会科研课题(20222121010688);黑龙江省中医药科研项目(ZHY2022-084)。

摘　　要：目的探讨柴术理胃饮对肝郁脾虚型功能性消化不良(functional dyspepsia,FD)大鼠胃肠动力及p-NF-κBp65通路的影响。方法60只SD大鼠按随机数字表法分为正常组(n=10)和模型储备组(n=50),模型储备组采用复合因素刺激法复制肝郁脾虚型FD模型,模型复制成功后将50只大鼠按随机数字表法分成模型组、中药高、中、低剂量组和多潘立酮组,每组各10只。各组分别给予相应的药物灌胃后,观察大鼠的一般状态;计算大鼠进食量、体质量、胃排空率和小肠推进率;酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)方法测定大鼠胃窦组织白细胞介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平;Western blot法检测和实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)方法检测大鼠胃窦组织中p-NF-κBp65蛋白表达及p-NF-κBp65 mRNA表达情况;HE染色观察胃窦组织形态。结果与正常组比较,模型组大鼠进食量、体质量、胃排空率和小肠推进率均减低,胃窦组织IL-6、TNF-α水平、p-NF-κBp65蛋白表达及p-NF-κBp65 mRNA表达均升高(P<0.05);与模型组比较,中药高、中、低剂量组和多潘立酮组大鼠进食量、体质量、胃排空率和小肠推进率均提高,胃窦组织IL-6、TNF-α水平、p-NF-κBp65蛋白表达及p-NF-κBp65 mRNA表达均降低(P<0.05);中药高剂量组IL-6、TNF-α水平低于多潘立酮组(P<0.05);中药高、中剂量组p-NF-κBp65蛋白表达低于多潘立酮组(P<0.05)。结论柴术理胃饮治疗肝郁脾虚型FD的机制可能与降低大鼠胃窦组织IL-6、TNF-α水平、p-NF-κBp65蛋白表达及p-NF-κBp65 mRNA表达,改善胃肠运动功能有关。Objective To investigate the effect of chaizhu liwei decoction on gastrointestinal motility and p-NF-κBp65 pathway in functional dyspepsia(FD)rats with liver depression and spleen deficiency.Methods Sixty SD rats were random number table method divided into normal group(n=10)and model reserve group(n=50).The FD model of liver depression and spleen deficiency was replicated by compound factor stimulation in the model reserve group.After successful replication of the model,50 rats were random number table method divided into model group,traditional Chinese medicine high-dose,medium-dose and low-dose groups and domperidone group,with 10 rats in each group.Each group was given corresponding drugs by gavage,and the general state of rats was observed.The food intake,body mass,gastric emptying rate and intestinal propulsion rate were calculated.The levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in gastric antrum tissue of rats were detected by enzyme-linked immunosorbent assay(ELISA).Western blot and real-time quantitative polymerase chain reaction(RT-qPCR)were used to detect the expression of p-NF-κBp65 protein and p-NF-κBp65 mRNA in gastric antrum tissue of rats.HE staining was used to observe the morphology of gastric antrum.Results Compared with the normal group,the food intake,body mass,gastric emptying rate and intestinal propulsion rate of rats in the model group were decreased,and the levels of IL-6,TNF-α,p-NF-κBp65 protein expression and p-NF-κBp65 mRNA expression in gastric antrum tissue were increased(P<0.05).Compared with the model group,the food intake,body mass,gastric emptying rate and intestinal propulsion rate of rats in the traditional Chinese medicine groups and domperidone group were increased,and the levels of IL-6,TNF-α,p-NF-κBp65 protein expression and p-NF-κBp65 mRNA expression in gastric antrum tissue were decreased(P<0.05).The levels of IL-6 and TNF-αin the traditional Chinese medicine high-dose group were lower than those in the domperidone group(P<0.05).The protein

关 键 词：功能性消化不良 肝郁脾虚型 柴术理胃饮 p-NF-κBp65通路 

分 类 号：R57[医药卫生—消化系统]