Meiotic transcriptional reprogramming mediated by cell-cell communications in humans and mice revealed by scATACseq and scRNA-seq  

作　　者：Hai-Quan Wang Xiao-Long Wu Jing Zhang Si-Ting Wang Yong-Juan Sang Kang Li Chao-Fan Yang Fei Sun Chao-Jun Li 

机构地区：[1]State Key Laboratory of Reproductive Medicine and Offspring Health,Center for Global Health,School of Public Health,Nanjing Medical University,Nanjing,Jiangsu 211166,China [2]Medical School,Nanjing University,Nanjing,Jiangsu 210093,China [3]Zhejiang University School of Medicine,Hangzhou,Zhejiang 310016,China [4]Department of Urology and Andrology,Sir Run Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou,Zhejiang 310000,China [5]Model Animal Research Center of Medical School,Nanjing University,Nanjing,Nanjing,Jiangsu 210093,China [6]Liangzhu Laboratory,Zhejiang University,Hangzhou,Zhejiang 311121,China

出　　处：《Zoological Research》2024年第3期601-616,共16页动物学研究（英文）

基　　金：supported by the National Natural Science Foundation of China(82271645);National Key Research and Development Program of China(2021YFC2700200 to F.S.)。

摘　　要：Meiosis is a highly complex process significantly influenced by transcriptional regulation.However,studies on the mechanisms that govern transcriptomic changes during meiosis,especially in prophase I,are limited.Here,we performed single-cell ATAC-seq of human testis tissues and observed reprogramming during the transition from zygotene to pachytene spermatocytes.This event,conserved in mice,involved the deactivation of genes associated with meiosis after reprogramming and the activation of those related to spermatogenesis before their functional onset.Furthermore,we identified 282 transcriptional regulators(TRs)that underwent activation or deactivation subsequent to this process.Evidence suggested that physical contact signals from Sertoli cells may regulate these TRs in spermatocytes,while secreted ENHO signals may alter metabolic patterns in these cells.Our results further indicated that defective transcriptional reprogramming may be associated with non-obstructive azoospermia(NOA).This study revealed the importance of both physical contact and secreted signals between Sertoli cells and germ cells in meiotic progression.

关 键 词：Single-cell RNA-seq Single-cell ATAC-seq SPERMATOGENESIS MEIOSIS Transcriptional reprogramming Cell-cell communication 

分 类 号：Q343[生物学—遗传学]