安徽省马鞍山市蜱携5种病原体的检测研究  

Detection of five tick-borne pathogens in Maanshan City,Anhui Province,China

作　　者：杨国栋 杨锟[3] 江良梁[3] 吴铭 洪颖[3] 项可霞[3] 何佳 龚磊[4] 宋丹丹[4] 包名家[5] 李兴洲[1] 秦天[2] 王艳华[2] YANG Guo-dong;YANG Kun;JIANG Liang-liang;WU Ming;HONG Ying;XIANG Ke-xia;HE Jia;GONG Lei;SONG Dan-dan;BAO Ming-jia;LI Xing-zhou;QIN Tian;WANG Yan-hua(College of Public Health&Jiamusi University,Heilongjiang Province,Jiamusi 154002,China;National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention/National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,Beijing 102206,China;Maanshan Center for Disease Control and Prevention,Anhui Province,Maanshan 243000,China;Anhui Center for Disease Control and Prevention,Hefei 230000,China;Jiamusi Center for Disease Control and Prevention,Heilongjiang Province,Jiamusi 154002,China)

机构地区：[1]黑龙江省佳木斯大学公共卫生学院,佳木斯154002 [2]中国疾病预防控制中心传染病预防控制所,传染病溯源预警与智能决策全国重点实验室,北京102206 [3]安徽省马鞍山市疾病预防控制中心,马鞍山243000 [4]安徽省疾病预防控制中心,合肥230000 [5]黑龙江省佳木斯市疾病预防控制中心,佳木斯154002

出　　处：《中国人兽共患病学报》2024年第4期308-314,共7页Chinese Journal of Zoonoses

基　　金：国家自然科学基金面上项目(No.81874275);北京市自然科学基金面上项目(No.7242188)联合资助。

摘　　要：目的了解安徽省马鞍山市蜱携5种重要病原体的感染情况,并分析其与我国既往检出病原体的亲缘关系。方法在马鞍山市周边13个村庄采蜱642只,采用形态学和线粒体COI基因鉴定蜱种。对于土拉弗朗西斯菌检测16S rRNA,巴尔通体检测ssrA,立克次体检测16S rRNA、ompA和ompB,无形体检测16S rRNA和gltA,柯克斯体检测groEL和rpoB。在公共数据平台选取有代表性的序列,与本研究测序获得的基因序列比对,采用MEGA11.0构建系统进化树。结果在640只长角血蜱中共有36只蜱检出立克次体,其中20只蜱中检出黑龙江立克次体,16只蜱中检出敬信立克次体,2只蜱中检出牛无形体,186只蜱中检出柯克斯体样内共生体。本研究检出的黑龙江立克次体HY2和安徽省B8菌株聚在一个分支,敬信立克次体QL3和山西、江苏的聚在一个分支,而牛无形体JX4和山西省的聚在一个分支。经分析,还有17只蜱存在合并感染。5种病原体在2只龟形花蜱中均没有检测到。结论本研究首次在安徽省长角血蜱中检出敬信立克次体。对于斑点热立克次体和牛无形体,建议当地卫生部门做好相应的防控措施。Here,5 important pathogens carried by ticks in Maanshan City,Anhui Province,China were identified.In total,642 ticks were collected from 13 villages around Maanshan City and identified by morphological and mitochondrial COI genes.The 16S rRNA gene of Francisella tularensis,ssrA gene of Bartonella,16S rRNA,ompA and ompB genes of Rickettsia,16S rRNA and gltA genes of Anaplasma,and groEL and rpoB genes of Coxiella were sequenced.Reference sequences were retrieved from a public database.Phylogenetic trees were constructed with MEGA11.0 software.In total,36 Rickettsiae isolates were detected in 640 Haemaphysalis longicornis ticks,which included 20 isolates of Rickettsia heilongjiangensis,16 of Candidatus Rickettsia jingxinensis,2 of Anaplasma bovis,and 186 of Coxiella-like endosymbiont.R.heilongjiangensis HY2 detected in this study and Anhui B8 strain,Ca.R.jingxinensis QL3 and those from Shanxi Province and Jiangsu Province,A.bovis JX4 and those from Shanxi Province were clustered on the same branch.Overall,17 ticks had combined infections and none of the 5 bacteria were detected in two Amblyomma testudinarium ticks.This is the first report of Ca.R.jingxinensis detected in H.longicornis ticks from Anhui Province.It is recommended that the two types of Rickettsia that cause spotted fever and A.bovis should be reported to local health authorities to initiate appropriate prevention and control measures.

关 键 词：立克次体 无形体 柯克斯体 长角血蜱 安徽省 系统进化分析 

分 类 号：R384.4[医药卫生—医学寄生虫学]