水稻优良育种材料Wx基因型鉴定及相关性状分析  

Identification of Wx Genotypes in Excellent Breeding Materials of Rice (Oryza Sativa L.) and Analysis of Related Traits

作　　者：郑英 艾妍雨 魏应海 彭言劼 罗李飞 务奇生 周勇 ZHENG Ying;AI Yanyu;WEI Yinghai;PENG Yanjie;LUO Lifei;WU Qisheng;ZHOU Yong(Leshan Academy of Agricultural Sciences,Sichuan Leshan 614000,China;School of Life Science/Institute ofBiodiversity Conservation and Utilization in Mount Emei,Leshan Normal University,Sichuan Leshan 614000,China;Leshan Seed Management Sation and Rural Bureau,Sichuan Leshan 614000,China;Leshan Agricultural Information Station,Sichuan Leshan 614000)

机构地区：[1]乐山市农业科学研究院,四川乐山614000 [2]乐山师范学院生命科学学院/峨眉山生物多样性保护与利用研究所,四川乐山614000 [3]乐山市种子管理站,四川乐山614000 [4]乐山市农业信息站,四川乐山614000

出　　处：《乐山师范学院学报》2024年第4期15-22,41,共9页Journal of Leshan Normal University

基　　金：四川省“十四五”农作物育种攻关项目“引领性水稻育种材料和方法创新及新品种选育”(2021YFYZ0016);国家级大学生创新创业训练项目“新育成的几个杂交稻不育系Wx基因型鉴定及表型分析”(S202010649087)。

摘　　要：为了鉴定优异育种材料的Waxy(Wx)基因型及其相关性状的表型,利用DNA分子标记,结合特异引物扩增片段测序,对27份水稻优异育种材料Wx基因第1内含子上游的(CT)n序列、第1内含子+1位G/T单核苷酸突变(single nucleotide polymorphism,SNP)、第4外显子A/G SNP和第6外显子的A/C SNP进行了鉴定;同时对直链淀粉含量(amylose content,AC)和胶稠度(gel consistency,GC)进行了测定。结果表明,27份水稻材料Wx基因第1内含子上游共有5种(CT)n多态性(n=10,11,12,17,18),包括Wxa型6份、Wxb型16份、Wxhp型3份、Wxin型1份和wx型1份;上述5种Wx基因型水稻对应的AC含量分别为23.1%~29.0%、10.9%~16.4%、5.7%~9.8%、18.4%和3.5%,(CT)n多态性和G/T SNP分别能解释88.9%和85.2%的直链淀粉含量变异,且(CT)n多态性标记具有较高的选择准确性;AC与GC间呈显著的负相关(r=-0.4827*),但也存在高直高胶和低直低胶的特异材料。研究结果对培育优良杂交稻亲本和组配优质杂交稻品种具有指导意义。In order to identify the Wx genotype and the phenotype of its related traits in the elite breeding materials,the(CT)n sequence upstream of the first intron of the Wx gene,the first intron+1 G/T SNP,the A/G SNP of exon 4 and the A/C SNP of exon 6 of 27 elite rice breeding materials were identified by DNA molecular markers combined with specific primer amplification fragment sequencing.Amylose content(AC)and gel consistency(GC)were also measured.The results showed that there were five(CT)n polymorphisms(n=10,11,12,17,18)upstream of the first intron of the Wx gene in the 27 rice materials.Six of them were of the Wxa genotype,16 were of the Wxb genotype,three were of the Wxhp genotype,one was of the Wxin genotype,and one was wx.Wxa materials had higher AC(23.1%~29.0%),Wxb materials had moderate AC(10.9%~16.4%),Wxhp materials had lower AC(5.7%~9.8%),Wxin materials had an AC of 18.4%,and wx materials were glutinous rice with an AC of 3.5%.(CT)n polymorphisms and G/T SNP explained 88.9%and 85.2%of the variation in amylose content,respectively,and(CT)n polymorphism markers had high selection accuracy.There was a significant negative correlation between AC and GC(r=-0.4827*),but there were also specific materials with high AC and high GC,or low AC and low GC.This study provides material and technical references for cultivating excellent hybrid rice parents,breeding high-quality and special hybrid rice varieties.

关 键 词：水稻 WX基因 直链淀粉含量 胶稠度 

分 类 号：S511[农业科学—作物学]