高直链淀粉玉米体外产气和营养物质降解参数评估  

Evaluation of Parameters of Gas Production and Nutrient Degradation of High Amylose Corn In Vitro

作　　者：伍志武 刘国威 陈凯 汪水平[3] 贺志雄[1,2] 谭支良 WU Zhiwu;LIU Guowei;CHEN Kai;WANG Shuiping;HE Zhixiong;TAN Zhiliang(South Central Experimental Station of Animal Nutrition and Feed Science in the Ministry of Agriculture,Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process,National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,CAS Key Laboratory for Agro-ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,The Chinese Academy of Sciences,Hunan Changsha 410125,China;University of Chinese Academy of Sciences,Beijing 100049,China;College of Animal Science,Southwest University,Chongqing 402460,China;College of Animal Science and Technology,Hunan Agricultural University,Hunan Changsha 410128,China)

机构地区：[1]中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验室,畜禽养殖污染控制与资源化技术国家工程实验室,湖南省动物营养生理与代谢过程重点实验室,农业部中南动物营养与饲料科学观测实验站,湖南长沙410125 [2]中国科学院大学,北京100049 [3]西南大学动物科学学院,重庆402460 [4]湖南农业大学动物科学技术学院,湖南长沙410128

出　　处：《饲料工业》2024年第11期85-90,共6页Feed Industry

基　　金：内蒙古揭榜挂帅项目[2022JBGS0023];湖南省自然科学基金项目[2022JJ10054];湖南创新型省份建设专项[2019RS3021]。

摘　　要：试验旨在利用体外人工瘤胃比较高直链淀粉玉米(简称高直玉米)与普通玉米体外产气和营养物质降解参数差异,为高直玉米在反刍动物中高效利用提供依据。试验设置高直玉米和普通玉米两个组,应用全自动体外模拟瘤胃发酵技术分别测定玉米发酵3、6、12、24 h气体产量、甲烷产量、干物质(DM)降解率和淀粉降解率,并利用非线性拟合函数计算产气量和营养物质降解模型参数。结果表明:①高直玉米的产气量和起始底物降解速率显著低于普通玉米(P<0.05),产气速率呈降低趋势(0.05<P<0.10);②高直玉米的甲烷产量和潜在最大产甲烷量显著低于普通玉米(P<0.05);③高直玉米瘤胃可降解DM消失速率常数和DM降解滞后期显著低于普通玉米(P<0.05);④高直玉米瘤胃可降解淀粉消失速率常数和淀粉降解滞后期显著低于普通玉米(P<0.05)。综上可知,与普通玉米相比,高直玉米在瘤胃内发酵缓慢、降解速率低,但随着滞留时间的延长,两者发酵模式会趋于一致。The experiment aimed to use an artificial rumen in vitro to compare the differences of parameters of gas production and nutrient degradation in vitro between high amylose corn and normal corn,providing a basis for the efficient utilization of high amylose corn in ruminants.The experiment consisted of two groups:high-amylose corn and normal corn.The fully automatic in vitro simulated rumen fermentation technique was applied to measure gas production,methane production,dry matter(DM)degradation rates,and starch degradation rates at 3,6,12 h and 24 h of fermentation.Nonlinear fitting functions were used to calculate gas production and nutrient degradation model parameters.The results showed that:①the gas production and initial substrate degradation rate of high amylose corn were significantly lower than those of normal corn(P<0.05),with a decreasing trend in gas production rate(0.05<P<0.10);②the methane production and potential maximum methane production of high amylose corn were significantly lower than those of normal corn(P<0.05);③the disappearance rate constant of rumen-degradable DM and the lag time of DM degradation for high amylose corn were significantly lower than those for normal corn(P<0.05);④the disappearance rate constant of rumen-degradable starch and lag time of starch degradation for high amylose corn were significantly lower than those for normal corn(P<0.05).In conclusion,compared with normal corn,high amylose corn exhibits slower rumen fermentation and lower degradation velocity.However,with prolonged retention time,the fermentation patterns of the two tend to converge.

关 键 词：高直链淀粉玉米 体外发酵 产气量 降解率 瘤胃发酵 

分 类 号：S816.32[农业科学—饲料科学]