基于米氏机理的酶催化反应中反应速率常数的计算  

Determination of All Rate Constants in the Enzyme Catalyzed Reactions Based on Michaelis-Menten Mechanism

作　　者：张恒[1] Heng Zhang(College of Chemistry and Molecular Sciences,Wuhan University,Wuhan 430072,China)

机构地区：[1]武汉大学化学与分子科学学院,武汉430072

出　　处：《大学化学》2024年第4期395-400,共6页University Chemistry

摘　　要：对基于米氏机理的酶催化反应模型,本文介绍了求解全部反应速率常数的两种方法:瞬态法和数学计算法,其中瞬态法的核心是分析反应达到稳态前的过程,数学计算法的核心是寻找反应过程中酶与底物浓度之间的关系。通过求解全部反应速率常数,不仅可以加深对酶催化反应动力学的理解,而且有助于提高对反应动力学的认识。Two protocols,the transient method and the mathematical method,are introduced to deduce all the rate constants in the enzyme-catalyzed reaction model based on the Michaelis-Menten mechanism.The transient method focuses on analyzing the period before reaching a steady state,while the mathematical method aims to establish a relationship between the concentration of the enzyme and the substrate.By calculating all the rate constants,one can enhance their understanding and grasp of the kinetics of the enzyme-catalyzed reaction.

关 键 词：酶催化反应 动力学 米氏机理 速率常数 

分 类 号：G64[文化科学—高等教育学] O6[文化科学—教育学]