茯苓酸对肾癌细胞上皮间质转化和免疫逃逸的影响  

Impacts of Poric Acid on Epithelial Mesenchymal Transition and Immune Escape in Renal Cancer Cells

作　　者：李伟博 何春艳 张玲玲 王静[2] 邸彦橙 Li Weibo;He Chunyan;Zhang Lingling;Wang Jing;Di Yancheng(Department of Urinary Surgery,Hongqi Hospital Affiliated to Mudanjiang Medical University,Mudanjiang 157011,China;School of Basic Medical,Mudanjiang Medical University,Mudanjiang 157011,China)

机构地区：[1]牡丹江医学院附属红旗医院泌尿外科,黑龙江牡丹江157011 [2]牡丹江医学院基础医学院,黑龙江牡丹江157011

出　　处：《南开大学学报（自然科学版）》2024年第2期22-27,共6页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：黑龙江省卫生健康委科研课题(2020-407)。

摘　　要：选取786-O细胞随机分为空白组、茯苓酸组、CXCL12组、CXCL12-CXCR4抑制剂-AMD3100组、茯苓酸+CXCL12组、茯苓酸+AMD3100组,CCK-8检测细胞增殖;Transwell检测细胞迁移、侵袭;免疫荧光检测EMT相关因子-E-钙粘素(E-Cadherin)、锌指转录因子(Snail)、N-钙黏素(N-Cadherin)的表达;与CD8+T细胞共培养,Elisa检测上清液中免疫逃逸相关因子-白细胞介素(IL)-4和IL-10、干扰素(IFN)-γ水平;Western blot检测CXCL12、CXCR4、CXCR7及免疫逃逸蛋白PD-L1表达.探讨茯苓酸调节CXCL12-CXCR4/CXCR7信号通路对肾癌细胞上皮间质转化(EMT)和免疫逃逸的影响.结果显示与空白组相比,茯苓酸组细胞增殖率、迁移、侵袭数、Snail、N-Cadherin IOD值、IL-10水平、CXCL12、CXCR4、CXCR7以及PD-L1表达显著降低,E-Cadherin IOD值、IFN-γ、IL-4水平显著增加(P<0.05);与茯苓酸组相比,茯苓酸+CXCL12组细胞增殖率、迁移、侵袭数、Snail、N-Cadherin IOD值、IL-10水平、CXCL12、CXCR4、CXCR7以及PD-L1表达显著增加,E-Cadherin IOD值、IFN-γ、IL-4水平显著降低(P<0.05);与CXCL12组相比,茯苓酸+CXCL12组细胞增殖率、迁移、侵袭数、Snail、N-Cadherin IOD值、IL-10水平、CXCL12、CXCR4、CXCR7以及PD-L1表达显著降低,E-Cadherin IOD值、IFN-γ、IL-4水平显著增加(P<0.05);与茯苓酸组、AMD3100组相比,茯苓酸+AMD3100组细胞增殖率、迁移、侵袭数、Snail、N-Cadherin IOD值、IL-10水平、CXCL12、CXCR4、CXCR7以及PD-L1表达显著降低,E-Cadherin IOD值、IFN-γ、IL-4水平显著增加(P<0.05).表明茯苓酸可调节CXCL12-CXCR4/CXCR7信号通路阻止肾癌细胞免疫逃逸及EMT.786-O cells were randomly separated into blank group,Poric acid group,CXCL12 group,CXCL12-CXCR4 inhibitor-AMD3100 group,Poric acid+CXCL12 group,and poric acid+AMD3100 group.CCK-8 was applied to detect cell proliferation.Transwell was applied to detect cell migration and invasion.Immunofluorescence was applied to detect the expression of EMT related factors such as E-Cadherin,zinc finger transcription factor(Snail),and N-cadherin.After co-culturing with CD8+T cells,Elisa was applied to detect the levels of immune escape related factors-interleukin-4(IL-4),IL-10,and interferon-γ(IFN-γ)in the supernatant.Western blot was applied to detect the expression of CXCL12,CXCR4,CXCR7,and immune escape protein PD-L1.To investigate the impacts of poric acid on epithelial mesenchymal transition(EMT)and immune escape in renal cancer cells by regulating the CXCL12-CXCR4/CXCR7 signaling pathway.Results showed that compared with the blank group,the proliferation rate,numbers of migration and invasion,the Snail,N-Cadherin IOD values,the level of IL-10,the expression of CXCL12,CXCR4,CXCR7,and PD-L1 in the Poria acid group were greatly reduced,the E-Cadherin IOD value,and the levels of IFN-γand IL-4 were greatly increased(P<0.05).Compared with the poria acid group,the proliferation rate,numbers of migration and invasion,the Snail,N-Cadherin IOD values,the level of IL-10,the expression of CXCL12,CXCR4,CXCR7,and PD-L1 in the poria acid+CXCL12 group were greatly increased,the E-Cadherin IOD value,and the levels of IFN-γand IL-4 were greatly reduced(P<0.05).Compared with the CXCL12 group,the proliferation rate,numbers of migration and invasion,the Snail,N-Cadherin IOD values,the level of IL-10,the expression of CXCL12,CXCR4,CXCR7,and PD-L1 were greatly reduced in the Poric acid+CXCL12 group,the E-Cadherin IOD value,and the levels of IFN-γand IL-4 were greatly increased(P<0.05).Compared with the Poria acid group and AMD3100 group,the proliferation rate,numbers of migration and invasion,the Snail,N-Cadherin IOD values,the level of IL-1

关 键 词：茯苓酸 CXCL12-CXCR4/CXCR7信号通路 肾癌细胞 免疫逃逸 上皮间质转化 

分 类 号：R737.11[医药卫生—肿瘤]