基于UPLC-ABTS-Q-TOF-MS的艾草不同部位抗氧化活性成分比较研究  

A comparative study of antioxidant active components in different parts of Artemisia argyi by UPLC-ABTS-Q-TOF-MS

作　　者：王苗苗 王宇卿 李淑娇[2] 庄果 杨媛媛 WANG Miao-miao;WANG Yu-qing;LI Shu-jiao;ZHUANG Guo;YANG Yuan-yuan(School of Pharmacy,Henan University,Kaifeng 475004,China;Key Laboratory of Traditional Chinese Medicine Effector and Quality Control,Institute of Nanyang Geoherbs(Artemisia argyi),Nanyang Medical College,Nanyang 473061,China)

机构地区：[1]河南大学药学院,河南开封475004 [2]南阳医学高等专科学校,河南省中药效应物质与质量控制研究中医药重点实验室,南阳道地药材(艾草)研究院,河南南阳473061

出　　处：《中国中药杂志》2024年第9期2468-2477,共10页China Journal of Chinese Materia Medica

基　　金：南阳市重大科技专项(2023ZDZX105);河南省重点研发与推广专项(242102310562);河南省高等学校重点科研项目(23B350007);南阳市科技攻关计划项目(KJGG106,23KJGG169);南阳市基础与前沿技术研究计划项目(JCQY023);河南省高校科技创新人才支持计划项目(19HASTIT007);河南省基础与前沿技术研究项目(152300410181)。

摘　　要：为了对中药艾Artemisia argyi不同部位(根、茎、叶、籽)中化学成分进行表征与鉴定,筛选具有抗氧化活性的化合物,该研究采用超高效液相色谱-2,2′-联氨-双(3-乙基苯并噻唑啉-6-磺酸)二胺盐-串联四极杆飞行时间质谱(UPLC-ABTS-Q-TOF-MS)在线联用技术,以Poroshell 120 SB-Aq(3.0 mm×150 mm,2.7μm)为色谱柱,乙腈(A)-0.2%的甲酸水(B)为流动相,进行梯度洗脱,在正、负离子模式下分别扫描,运用MassLynx软件,结合对照品及相关文献,对艾草不同部位进行化学成分鉴定并比较不同部位化学成分的差异,采用抗氧化活性成分在线检测体系,对抗氧化活性成分进行检测,并以清除效率为指标对艾草不同部位活性成分的抗氧化活性进行比较评价。结果从艾草不同部位提取物中共鉴定出87个化合物,根、茎、叶、籽分别鉴定出38、72、85、33个成分;共筛选出具有22个抗氧化活性的化合物,根、茎、叶、籽分别鉴定出14、17、20、11个具有抗氧化活性的化合物。研究表明艾草不同部位化学成分和抗氧化成分均存在一定差异,为艾草的资源利用和进一步研究开发提供数据支持。In order to characterize and identify the chemical components in different parts of Artemisia argyi(roots, stems, leaves, and seeds), compounds with antioxidant activity were screened. In this study, ultra-performance liquid chromatography-2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt-quadrupole time-of-flight-tandem mass spectrometry(UPLC-ABTS-Q-TOF-MS) was used as an online combination technique. Poroshell 120 SB-Aq(3.0 mm×150 mm, 2.7 μm) was used as the column, and acetonitrile(A)-0.2% formic acid water(B) was adopted as the mobile phase to perform gradient elution and was scanned in positive and negative ion modes. MassLynx software was utilized, and combined with reference substances and related literature, the chemical components of different parts of A. argyi were identified and compared. The antioxidant active components were detected by using the online detection system, and the antioxidant activities of active components of different parts of A. argyi were compared and evaluated by scavenging efficiency. As a result, a total of 87 compounds were identified from extracts of different parts of A. argyi, and 38, 72, 85, and 33 components were identified from roots, stems, leaves, and seeds. 22 compounds with antioxidant activity were screened, and 14, 17, 20, and 11 compounds with antioxidant activity were identified from roots, stems, leaves, and seeds. The results show that there are certain differences in chemical components and antioxidant components of different parts of A. argyi, which provides data support for the resource utilization and further research and development of A. argyi.

关 键 词：艾草 在线筛选 抗氧化活性 UPLC-ABTS-Q-TOF-MS 2 2′-联氨-双(3-乙基苯并噻唑啉-6-磺酸)二胺盐 

分 类 号：R284.1[医药卫生—中药学] O657.63[医药卫生—中医学]