ATP4A联合ATP4B可作为胃癌早期诊断及判断预后的生物标志物  

ATP4A combined with ATP4B can be used as a biomarker for early diagnosis and prognosis of gastric cancer

作　　者：黎滨茹 李琳琳[1] 马锐[1] LI Bin-ru;LI Lin-lin;MA Rui(Department of Thoracic Medicine,Liaoning Cancer Hospital,Dalian University of Technology,Shenyang 110042,China)

机构地区：[1]大连理工大学附属肿瘤医院、辽宁省肿瘤医院胸内科,辽宁沈阳110042

出　　处：《解剖科学进展》2024年第2期153-156,160,共5页Progress of Anatomical Sciences

基　　金：国家自然科学基金(81901846)。

摘　　要：目的利用生物信息学筛选出潜在的具有早期诊断及判断胃癌预后的生物标志物。方法应用GEO2R从GEO数据集筛选出差异表达基因,应用GO/KEGG进行功能富集分析,使用STRING工具生成蛋白互作网络,再利用Cytoscape的MCODE插件鉴定出Hub基因。使用HPA分析Hub基因在胃癌组织中的表达情况,基于Kaplan-Meier分析预后生存曲线。结果从GSE13911、GSE19826、GSE29272三个数据集得到105个有交集的DEGs,它们主要参与代谢途径。筛选出蛋白互作节点共75个,最后得到10个Hub基因,其中发现ATP4A和ATP4B有明显相关性(r=0.699,P<0.001),且发现ATP4A和ATP4B在胃癌组织细胞浆中表达升高,与预后差呈正相关。结论ATP4A和ATP4B可能作为胃癌潜在的早期诊断及判断预后的生物标志物。Objective To screen potential biomarkers for early diagnosis and prognosis of gastric cancer by bioinformatics.Methods GEO2R was used to screen differentially expressed genes from GEO data set,and GO/KEGG was used for functional enrichment analysis.The STRING tool was used to generate the protein interaction network,and the hub genes were identified using the MCODE plugin of Cytoscape.The expression of Hub gene in gastric cancer tissues was analyzed using HPA,and the prognosis and survival curve was analyzed based on Kaplan-Meier.Results 105 intersecting DEGs were obtained from three datasets GSE13911,GSE19826 and GSE29272,which were mainly involved in metabolic pathways.A total of 75 protein interaction nodes were screened,and 10 Hub genes were obtained,among which ATP4A and ATP4B were significantly correlated(r=0.699,P<0.001).The expressions of ATP4A and ATP4B in the cytoplasm of gastric cancer tissue were increased,and were positively correlated with poor prognosis.Conclusion ATP4A and ATP4B may be potential biomarkers for early diagnosis and prognosis of gastric cancer.

关 键 词：胃癌 Hub基因 ATP4A ATP4B GEO数据库 

分 类 号：R735.2[医药卫生—肿瘤]