FERMT1通过NF-κB信号通路调控前列腺癌细胞增殖、迁移、侵袭及凋亡的实验研究  

Experimental study of FERMT1 affecting the proliferation,migration,invasion and apoptosis of prostate cancer cells by regulating the NF-κB signaling pathway

作　　者：张悦[1] 于学炜[1] 彭大振 于宏川[1] 仲伟一[1] ZHANG Yue;YU Xue-wei;PENG Da-zhen;YU Hong-chuan;ZHONG Wei-yi(Department of Urology,the Third People's Hospital of Dalian City,Dalian 116031,China)

机构地区：[1]大连市第三人民医院泌尿外科,辽宁大连116031

出　　处：《解剖科学进展》2024年第2期165-169,共5页Progress of Anatomical Sciences

基　　金：大连市卫生局科研基金(2016-0258X)。

摘　　要：目的探讨FERMT1对前列腺癌细胞增殖、迁移、侵袭、凋亡的影响及其对NF-κB信号通路的调控作用。方法采用qRT-PCR法与Western blot法分别检测正常人前列腺上皮细胞P69与前列腺癌细胞LNCaP、DU145、PC-3中FERMT1的表达量;si-NC、si-FERMT1、pcDNA-NC、pcDNA-FERMT1分别转染入LNCaP细胞,si-FERMT1转染入LNCaP细胞后加入NF-κB活化激活剂TNF-α处理细胞;采用MTT实验检测细胞活力;采用流式细胞术检测细胞凋亡率;采用Transwell小室实验检测细胞迁移及侵袭;采用Western blot法检测CyclinD1、MMP2、MMP9、Cleaved-caspase-3、p-p65、p-IκBα蛋白表达量。结果FERMT1在前列腺癌细胞中表达量升高,干扰FERMT1表达可抑制细胞增殖、迁移、侵袭及CyclinD1、MMP2、MMP9、p-p65、p-IκBα的表达,促进细胞凋亡及Cleaved-caspase-3的表达;FERMT1过表达可促进细胞增殖、迁移、侵袭及CyclinD1、MMP2、MMP9、p-p65、p-IκBα的表达,而抑制细胞凋亡及Cleaved-caspase-3的表达;NF-κB活化激活剂TNF-α处理细胞后能够逆转干扰FERMT1表达对细胞生物学行为的作用。结论干扰FERMT1的表达能够减弱前列腺癌细胞增殖、迁移及侵袭能力,促进细胞凋亡,其作用机制与抑制NF-κB信号通路的活化有关。Objective To explore the effect of FERMT1 on the proliferation,migration,invasion and apoptosis of prostate cancer cells and its regulation of NF-κB signaling pathway.Methods qRT-PCR and Western blot were used to detect the expression of FERMT1 in normal prostate epithelial cells P69 and prostate cancer cells LNCaP,DU145,and PC-3.si-NC,si-FERMT1,pcDNA-NC,and pcDNA-FERMT1 were transfected into LNCaP cells.After si-FERMT1 was transfected into LNCaP cells,NF-κB activation activator TNF-αwas added to treat the cells.MTT test was used to detect cell viability.Flow cytometry was used to detect the apoptosis rate.The transwell assay was used to detect cell migration and invasion.Western blot was used to detect the expressions of CyclinD1,MMP2,MMP9,Cleaved-caspase-3,p-p65,and p-IκBαprotein.Results The expression of FERMT1 was increased in prostate cancer cells.Interfering with the expression of FERMT1 could inhibit cell proliferation,migration,invasion and the expressions of CyclinD1,MMP2,MMP9,p-p65,p-IκBα,and promote cell apoptosis and the expression of Cleaved-caspase-3.Overexpression of FERMT1 could promote cell proliferation,migration,invasion and the expressions of CyclinD1,MMP2,MMP9,p-p65,p-IκBα,and inhibit cell apoptosis and the expression of Cleaved-caspase-3.NF-κB activation activator TNF-αcould reverse the effect of interfering with the expression of FERMT1 on the biological behavior of cells after treating the cells.Conclusion Interfering with the expression of FERMT1 could reduce the proliferation,migration and invasion of prostate cancer cells,and promote cell apoptosis,its mechanism of action was related to the inhibition of the activation of the NF-κB signaling pathway.

关 键 词：FERMT1 NF-ΚB信号通路 前列腺癌 增殖 迁移 侵袭 凋亡 

分 类 号：R737.25[医药卫生—肿瘤]