miR-129-5p靶向KLK7对卵巢癌SKOV3细胞增殖和侵袭的影响及分子机制  

The effect and molecular mechanism of miR-129-5p targeting KLK7 on the proliferation and invasion of ovarian cancer SKOV3 cells

作　　者：刘海忠[1] 韩玉花 任灵[2] 黄志 LIU Hai-zhong;HAN Yu-hua;REN Ling;HUANG Zhi(Department of Obstetrics and Gynecology,Chengdu Second People's Hospital,Chengdu 610017;Department of Pediatrics,Second Affiliated Hospital of Shenyang Medical College,Shenyang 110002;Department of Neurology,Sichuan First Veterans Hospital,Chengdu 610017,China)

机构地区：[1]成都市第二人民医院妇产科,四川成都610017 [2]沈阳医学院附属第二医院儿科,辽宁沈阳110002 [3]四川省第一退役军人医院神经内科,四川成都610017

出　　处：《解剖科学进展》2024年第2期186-189,共4页Progress of Anatomical Sciences

基　　金：辽宁省科学技术计划项目(2020-MS-311)。

摘　　要：目的探讨高表达miR-129-5p对卵巢癌SKOV3细胞增殖和侵袭的影响及分子机制。方法采用实时荧光定量PCR方法检测卵巢癌SKOV3细胞与人正常卵巢上皮细胞株中miR-129-5p的表达。在卵巢癌SKOV3细胞中瞬时转染miR-129-5p模拟物及无意义序列,采用CCK8法及Transwell实验检测各组细胞的增殖能力和侵袭能力;实时荧光定量PCR检测miR-129-5p和KLK7的相对表达水平;Western blot检测各组细胞中KLK7蛋白表达;Targetscan 7.2和双荧光素酶实验分析miR-129-5p与KLK7的靶向关系。结果miR-129-5p在卵巢癌SKOV3细胞中的表达低于人正常卵巢上皮细胞(P<0.05),过表达miR-129-5p降低SKOV3细胞中KLK7 mRNA和蛋白的表达,Targetscan 7.2网站分析及双荧光素酶实验证实KLK7为miR-129-5p的靶基因,过表达miR-129-5p抑制SKOV3细胞增殖和侵袭能力(P<0.05)。结论过表达miR-129-5p可通过靶向调控KLK7抑制卵巢癌SKOV3细胞增殖和侵袭。Objective To explore the effects and mechanisms of high expression of miR-129-5p on the proliferation and invasion of ovarian cancer SKOV3 cells.Methods qRT-PCR was used to detecte the expression of miR-129-5p in SKOV3 cells and IOSE80 cells.miR-129-5p mimics was transfected into SKOV3 cells,the expression of miR-129-5p and KLK7 in each group were detected by qRT-PCR,the expression of KLK7 was detected by Western blot,the targeting relationship between KLK7 and miR-129-5p was analyzed by targetscan 7.2 and double luciferase experiment,the proliferation and invasion of cells was detected by CCK8 and Transwell.Results The expression of miR-129-5p in SKOV3 cells was lower than that in IOSE80 cells(P<0.05).Over expression of miR-129-5p decreased the expression of KLK7 in SKOV3 cells(P<0.05).Targetscan 7.2 analysis and double luciferase experiment confirmed that KLK7 was the target gene of miR-129-5p.Over expression of miR-129-5p could inhibit the proliferation and invasion of SKOV3 cells(P<0.05).Conclusion Overexpression of miR-129-5p could inhibit the proliferation and invasion of ovarian cancer SKOV3 cells by targeting KLK7.

关 键 词：miR-129-5p SKOV3细胞 增殖 侵袭 

分 类 号：R737.31[医药卫生—肿瘤]