YAP调控LPS诱导的巨噬细胞M1型极化  

YAP regulates LPS-induced M1 macrophage polarization

作　　者：岳佩佩 邹博[1] 张珂鑫 朱晨[2] 吴彦菊 王彪[1] 孟欣 YUE Pei-pei;ZOU Bo;ZHANG Ke-xin;ZHU Chen;WU Yan-ju;WANG Biao;MENG Xin(Department of Biochemistry and Molecular Biology,College of Life Science,Shenyang 110122;Department of Neurosurgery of the First Hospital,China Medical University,Shenyang 110001,China)

机构地区：[1]中国医科大学生命科学学院生物化学与分子生物学教研室,辽宁沈阳110122 [2]中国医科大学附属第一医院神经外科,辽宁沈阳110001

出　　处：《解剖科学进展》2024年第2期197-200,204,共5页Progress of Anatomical Sciences

基　　金：国家自然科学基金(81572831);沈阳市科技计划项目(18-013-0-56)。

摘　　要：目的探讨YAP对脂多糖(Lipopolysaccharide,LPS)诱导的巨噬细胞M1型极化的影响。方法LPS诱导THP-1和U937细胞来源的M0型巨噬细胞向M1型极化。RT-qPCR法检测M1型巨噬细胞的标志物,罗丹明-鬼笔环肽检测细胞骨架,Western blot、RT-qPCR检测Hippo通路相关蛋白及其靶基因的表达。降低M0型巨噬细胞内YAP的表达,RT-qPCR检测LPS再诱导的M1型巨噬细胞的标志物的表达变化。结果在M0型巨噬细胞向M1型极化过中,Hippo通路关键因子YAP的蛋白水平升高,下游靶基因的表达也升高;敲低YAP或使用维替泊芬抑制YAP表达后,LPS再诱导的M1型巨噬细胞标志物的表达明显降低。结论YAP可以调控LPS诱导的巨噬细胞向M1极化。Objective To observe the effect of YAP on macrophage M1 polarization induced by LPS(Lipopolysaccharide).Methods PMA induces THP-1 and U937 cells to differentiate into macrophages(M0),then LPS continues to induce polarization to M1,RT-qPCR detected M1 marker,Rhodamine phalloidin was used to detect the changes of cytoskeleton.Western blot and RT-qPCR were used to detect the changes of Hippo pathway associated proteins and target genes.LPS induced M0 after reducing YAP expression,RT-qPCR was used to detect M1 markers changes.Results Western blot and RT-qPCR showed that the protein level of YAP and its target genes increased during the polarization of macrophages to M1;M1 markers were reduced after siYAP or treating cells with verteporfin compared to the control group.Conclusion YAP regulates LPS-induced macrophage polarization to M1.

关 键 词：巨噬细胞 M1 极化 YAP 

分 类 号：R730.2[医药卫生—肿瘤]