AbnI:Anα-ketoglutarate-dependent dioxygenase involved in brassicicene C-H functionalization and ring system rearrangement  

作　　者：Wenling Yuan Fengli Li Zhe Chen Qiaoxin Xu Zhenhua Guan Nanyu Yao Zhengxi Hu Junjun Liu Yuan Zhou Ying Ye Yonghui Zhang 

机构地区：[1]Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation,School of Pharmacy,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China

出　　处：《Chinese Chemical Letters》2024年第5期213-217,共5页中国化学快报（英文版）

基　　金：financially supported by the National Key R&D Program of China(Nos.2021YFA0910500,2021YFA0910503);the National Natural Science Foundation of China(Nos.22277035,32000045 and 81973205);the Program for Changjiang Scholars of Ministry of Education of the People’s Republic of China(No.T2016088);the National Natural Science Foundation for Distinguished Young Scholars(No.81725021);the National Science and Technology Project of China(No.2018ZX09201001-001-003);Innovative Research Groups of the National Natural Science Foundation of China(No.81721005);the Fundamental Research Funds for the Central Universities(No.2020kfyXJJS043).

摘　　要：We reported the characterization of a novel brassicicene diterpene biosynthetic gene cluster,which contains a uniqueα-ketoglutarate-dependent dioxygenase(αKGD)enzyme,AbnI.Our findings revealed that AbnI demonstrates remarkable substrate promiscuity and is capable of activating multiple sites on both 5-8-5 and 5-9-5 brassicicene skeletons,resulting in skeleton modifications and an unexpected ring system rearrangement.These results suggested the potential utility of AbnI as an enzymatic tool for terpene C-H functionalization.In addition,the catalytic mechanism of AbnI and its potential ecological implications were discussed.

关 键 词：Fungal diterpenes α-Ketoglutarate-dependent dioxygenase C-H functionalization REARRANGEMENT Brassicicenes 

分 类 号：TQ925[轻工技术与工程—发酵工程]