小麦根内细菌群落与黄花叶病发生的关联机制  

The relationship between the bacterial community in wheat root and the occurrence of wheat yellow mosaic disease

作　　者：张锐彬 秦方锦 张昊青 吴传发 祝贞科 葛体达 羊健 陈剑平 李科林 ZHANG Ruibin;QIN Fangjin;ZHANG Haoqing;WU Chuanfa;ZHU Zhenke;GE Tida;YANG Jian;CHEN Jianping;LI Kelin(School of Environmental Science and Engineering,Central South University of Forestry and Technology,Changsha 410004,China;Ningbo Agricultural Technology Popularization Station,Ningbo 315211,China;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Institute of Plant Virology,Ningbo University,Ningbo 315211,China)

机构地区：[1]中南林业科技大学环境科学与工程学院,长沙410004 [2]宁波市农业技术推广总站,宁波315211 [3]宁波大学植物病毒学研究所,省部共建农产品质量安全危害因子与风险防控国家重点实验室,农业部/浙江省植保生物技术重点实验室,宁波315211

出　　处：《植物病理学报》2024年第2期398-409,共12页Acta Phytopathologica Sinica

基　　金：宁波市科技局:大田土壤地力提升与养分资源高效利用技术集成与示范(2021Z101);财政部和农业农村部国家现代产业技术体系-小麦(CARS-03)资助。

摘　　要：植物根系微生物组在宿主生长发育、养分吸收以及病害抵御等方面发挥重要作用。为探究小麦根系微生物组变化与黄花叶病发生的关联机制,进而寻求小麦黄花叶病的生物防治方法,本研究采集了健康(H)、中度发病(M)和重度发病(S)小麦根系样品,研究了不同发病程度下小麦根内细菌群落结构及其环境驱动机制。结果表明:随着发病程度的增加,小麦根内细菌群落丰富度呈下降趋势。细菌群落结构在不同发病程度之间显著分异,且与根际土壤养分含量变化有关。其中短小杆菌属(Curtobacterium)、根瘤菌属(Rhizobium)、中慢生根瘤菌属(Mesorhizobium)、鞘氨醇单胞菌属(Sphingomonas)、人藤黄色杆菌属(Luteibacter)在发病小麦根内富集,其相对丰度与根际土壤中硝态氮(NO-3-N)、有效磷(AP)、有机碳(SOC)、全磷(TP)、全钾(TK)、植物体内碳(PC)含量呈显著正相关,与根际土壤中铵态氮(NH+4-N)、镁(Mg)含量呈显著负相关。泛菌属(Pantoea)在健康小麦根内富集,其相对丰度与植物体内氮(PN)含量呈显著正相关。以上结果表明,小麦根内细菌群落结构变化与小麦黄花叶病的发生及根际土壤养分含量增加有关。Microbial communities within plant root systems play significant roles in host growth and development,nutrient absorption,and disease resistance.To investigate the correlation between changes in the wheat root microbiome and the occurrence of wheat yellow mosaic,and consequently seek biological control methods for wheat yellow mosaic,this study collected wheat root samples that are healthy(H),moderately infected(M),and severely infected(S).The bacterial community structures within the wheat roots under varying degrees of infection and their environmental driving mechanisms were studied.Results revealed that as the degree of infection increased,the richness of bacterial communities within the wheat roots showed a declining trend.Bacterial community structures significantly differentiated among varying degrees of infection and were related to changes in the nutrient content of rhizosphere soil.Specifically,the genera Curtobacterium,Rhizobium,Mesorhizobium,Sphingomonas,and Luteibacter were enriched in infected wheat roots,and their relative abundance was positively correlated with the nitrate nitrogen(NO-3-N),available phosphorus(AP),organic carbon(SOC),total phosphorus(TP),total potassium(TK),and plant carbon(PC)content in the rhizosphere soil,but negatively correlated with ammonium nitrogen(NH+4-N)and magnesium(Mg)content.The genus Pantoea was enriched in healthy wheat roots,and its relative abundance was positively correlated with plant nitrogen(PN)content.These findings indicate that changes in the bacterial community structure within wheat roots are related to the occurrence of wheat yellow mosaic and the increase in nutrient content in the rhizosphere soil.

关 键 词：小麦黄花叶病 根内细菌 微生物多样性 高通量测序 

分 类 号：S158[农业科学—土壤学]