大蒜素对COPD大鼠肺组织损伤及内质网应激的影响  

The Effect of Allicin on Lung Tissue Damage and Endoplasmic Reticulum Stress in COPD Rats

作　　者：陈晓娟 覃文俊 Xiaojuan Chen;Wenjun Qin(Harbin Medical University,Harbin,Heilongjiang 150076)

机构地区：[1]哈尔滨医科大学,黑龙江哈尔滨150076

出　　处：《医学研究前沿》2024年第3期14-17,共4页

摘　　要：本研究的目的是探讨大蒜素(ALL)对慢性阻塞性肺疾病(COPD)大鼠肺组织损伤及内质网应激(ERS)的影响。我们采用了烟雾暴露法建立COPD大鼠模型,并将40只大鼠随机分为四组:空气暴露对照组、COPD模型组、COPD+低剂量ALL治疗组以及COPD+高剂量ALL治疗组。ALL的处理剂量分别为50mg/kg和100mg/kg,每天通过腹腔注射给药。治疗持续4周,之后对大鼠进行肺功能测试,并收集肺组织进行组织学分析和生化检测。结果显示,与COPD模型组相比,ALL治疗组的大鼠肺功能显著改善,肺组织病理损伤得到减轻。通过免疫组织化学染色和西方印迹分析,我们发现ALL能够显著降低COPD大鼠肺组织中内质网应激标志物GRP78和CHOP的表达。此外,ALL治疗显著减少了炎症细胞浸润和氧化应激水平,同时提高了抗氧化酶(如SOD和CAT)的活性。低剂量和高剂量ALL治疗组之间的比较显示,高剂量ALL的效果更为显著。由此可见,大蒜素对COPD大鼠模型的肺功能有显著的改善作用,并能减轻肺组织损伤。这可能与其降低内质网应激和抗炎症的作用有关。这为大蒜素在COPD治疗中的应用提供了实验基础。The purpose of this study is to investigate the effects of allicin(ALL)on lung tissue damage and endoplasmic reticulum stress(ERS)in rats with chronic obstructive pulmonary disease(COPD).We established a COPD rat model using smoke exposure method and ran-domly divided 40 rats into four groups:air exposure control group,COPD model group,COPD+low-dose ALL treatment group,and COPD+high-dose ALL treatment group.The treatment doses for ALL are 50mg/kg and 100mg/kg,respectively,administered daily via intraperito-neal injection.The treatment lasted for 4 weeks,followed by lung function testing on rats and collection of lung tissue for histological analy-sis and biochemical testing.The results showed that compared with the COPD model group,the ALL treatment group significantly improved lung function and reduced pathological damage to lung tissue in rats.Through immunohistochemical staining and Western blot analysis,we found that ALL can significantly reduce the expression of endoplasmic reticulum stress markers GRP78 and CHOP in the lung tissue of COPD rats.In addition,ALL treatment significantly reduced inflammatory cell infiltration and oxidative stress levels,while increasing the activity of antioxidant enzymes such as SOD and CAT.The comparison between low-dose and high-dose ALL treatment groups showed that high-dose ALL had a more significant effect.It can be seen that allicin has a significant improvement effect on lung function in COPD rat models and can alleviate lung tissue damage.This may be related to its role in reducing endoplasmic reticulum stress and anti-inflammatory effects.This provides an experimental basis for the application of allicin in the treatment of COPD.

关 键 词：大蒜素 COPD 大鼠肺组织损伤 

分 类 号：R563.8[医药卫生—呼吸系统]