多组学分析LOXL2在肺腺癌诊断和预后评估中的作用  

Multi-omics analysis of the role of lysyl oxidase-like 2 in the diagnosis and prognosis assessment of lung adenocarcinoma

作　　者：覃春莹 方灵燕 刘旻雁 粟永俊[1] 李晓莹 罗米兰 周英群[1] 韦常宏 QIN Chunying;FANG Lingyan;LIU Minyan;SU Yongjun;LI Xiaoying;LUO Milan;ZHOU Yingqun;WEI Changhong(Department of Medical Laboratory,the First People's Hospital of Nanning,Guangxi Zhuang Autonomous Region,Nanning530022,China)

机构地区：[1]南宁市第一人民医院医学检验科,广西南宁530022

出　　处：《中国当代医药》2024年第16期15-19,25,共6页China Modern Medicine

基　　金：广西壮族自治区卫生健康委员会自筹经费科研课题(Z20210959);广西壮族自治区临床重点专科建设项目经费资助;广西医科大学青年科学基金项目(GXMUYSF202328)。

摘　　要：目的采用多数据库深入分析赖氨酰氧化酶样蛋白2(LOXL2)在肺腺癌中的表达、基因组改变、预后和免疫浸润,以探讨其在肺腺癌诊断和预后评估中的作用。方法采用TIMER、UALCAN、GEPIA和Kaplan-Meier数据库分析LOXL2表达并进行生存分析。采用cBioPortal数据库分析LOXL2在肺腺癌患者中的基因组变化。采用TIMER分析LOXL2和免疫细胞浸润及免疫检查点的相关性。采用HPA数据库描述LOXL2在正常肺组织和肺腺癌组织的蛋白表达。结果LOXL2在肺腺癌中的mRNA和蛋白水平表达均高于正常组织。LOXL2的高表达与肺腺癌较晚的分期和较差的预后相关。LOXL2的基因改变率为6%。LOXL2与肺腺癌中的部分免疫细胞浸润和免疫检查点[程序性细胞死亡蛋白-1(PD-1)和细胞毒性T淋巴细胞相关抗原4(CTLA-4)]密切相关。免疫组织化学实验表明LOXL2在肺腺癌中的蛋白表达高于正常肺组织。结论通过多组学数据挖掘表明,LOXL2在肺腺癌组织中呈高表达,且与免疫细胞浸润和患者预后不良相关,LOXL2可作为肺腺癌诊断和预后的新型生物标志物。Objective To investigate the role of lysyl oxidase-like 2(LOXL2)in the diagnosis and prognosis of lung adenocarcinoma by analyzing the expression,genomic changes,prognosis and immune infiltration of LOXL2 in multiple databases.Methods LOXL2 expression was analyzed by TIMER,UALCAN,GEPIA and Kaplan-Meier databases and survival analysis was performed.Genomic changes of LOXL2 in lung adenocarcinoma patients were analyzed using cBioPortal database.The correlation between LOXL2 and immune cell infiltration and immune checkpoint was analyzed by TIMER.The protein expression of LOXL2 in normal lung tissue and lung adenocarcinoma tissue was described using HPA database.Results The mRNA and protein levels of LOXL2 in lung adenocarcinoma were higher than those in normal tissues.High expression of LOXL2 was associated with a later stage and poorer prognosis of lung adenocarcinoma.The rate of genetic change in LOXL2 was 6%.LOXL2 was strongly associated with partial immune cell infiltration and immune checkpoints(programmed cell death protein-1[PD-1]and cytotoxic T lymphocyte-associated antigen 4[CTLA-4])in lung adenoma.Immunohistochemistry showed that the protein expression of LOXL2 in lung adenocarcinoma was higher than that in normal lung tissue.Conclusion Through multi-omics data mining,LOXL2 is highly expressed in lung adenocarcinoma tissues,and is associated with immune cell infiltration and poor prognosis of patients.LOXL2 can be used as a novel biomarker for diagnosis and prognosis of lung adenocarcinoma.

关 键 词：肺腺癌 赖氨酰氧化酶样蛋白2 多组学 免疫浸润 预后 

分 类 号：R734.2[医药卫生—肿瘤]