不同药剂处理对黑芸豆种子表面细菌群落和萌发的影响  

Effects of Different Chemical Treatments on Surface Bacterial Community and Germination of Black Kidney Bean Seeds

作　　者：唐秀丽 杨建敏 孟志龙 Tang Xiuli;Yang Jianmin;Meng Zhilong(College of Biological Sciences and Technology,Taiyuan Normal University,Jinzhong,030619)

机构地区：[1]太原师范学院生物科学与技术学院,晋中030619

出　　处：《分子植物育种》2024年第11期3696-3704,共9页Molecular Plant Breeding

基　　金：山西省重点研发项目(201603D221008-1);山西省教育厅高校科技创新项目(2020L0523);山西省“三区”人才支持计划共同资助。

摘　　要：种子消毒是预防苗期病害、培育健壮幼苗必不可少的措施。本研究以黑芸豆种子为试材,利用Illumina MiSeq测序技术检测经3种不同药剂处理种子后,表面细菌群落多样性和结构特征以及潜在病原菌的丰度变化;测定种子发芽率、发芽势、发芽指数、胚根、胚轴、干重和鲜重等萌发指标,为科学选择黑芸豆种子的消毒药剂提供数据支持。结果表明:黑芸豆种子表面细菌群落共检测到21门41纲65目128科215属,变形菌门(Proteobacteria)(90.59%)为第一优势门,其次为放线菌门(Actinobacteria)(7.22%)和拟杆菌门(Bacteroidetes)(0.73%)。不同药剂处理的种子表面细菌群落多样性和丰富度存在差异,潜在病原菌Xanthomonas、Curtobacterium、Pantoea、Ralstonia和Pectobacterium相对丰度明显下降。3%中生菌素水剂效果最好,与CK相比,Pectobacterium丰度下降100%(P<0.01);Ralstonia丰度下降0.79%(P<0.001);Xanthomonas丰度下降3.05%(P<0.01);Curtobacterium丰度下降0.98%;Pantoea丰度下降0.76%。药剂处理后,黑芸豆种子发芽率、发芽势、发芽指数、胚根、胚轴、干重和鲜重较CK中均明显提高,不同药剂处理间存在差异。3%中生菌素处理后的种子萌发各指标变化最明显,与对照相比,发芽率提高了11.94%,发芽势提高了16.55%,胚根、胚轴分别增长3.50 cm、2.48 cm。干重、鲜重分别增加1.58 g、2.79 g,与对照差异显著(P<0.05)。Seed sterilization is an essential measure to prevent seedling diseases and cultivate healthy seedlings.In this study,the diversity and structural characteristics of surface bacterial communities and the abundance change of potential pathogens of black kidney bean seeds after treated by three different agents were detected using Illumina Mi Seq sequencing technology.And the germination indexes including seed germination rate,germination potential,germination index,radicle,hypocotyl,dry weight and fresh weight were measured.It will provide data support for scientific selection of disinfectants for black kidney bean seeds.The results showed that:21 phyla,41 classes,65 orders,128 families and 215 genera were detected in the surface bacterial community.Proteobacteria(90.59%)were the first dominant phyla,followed by Actinobacteria(7.22%)and Bacteroidetes(0.73%).There were differences in the diversity and richness of bacterial communities among three chemical treatments.The relative abundance of Xanthomonas,Curtobacterium,Pantoea,Ralstonia and Pectobacterium decreased significantly.3%Zhongshengmycin was the best seed treatment,the abundance of Pectobacterium,Ralstonia and Xanthomonas decreased significantly by 100%,0.79%and 3.05%respectively.The abundance of Curtobacterium and Pantoea decreased by 0.98%and0.76%respectively.The germination index of black kidney bean seeds and growth index of seedlings were significantly higher than those of CK after chemical treatment,and there were differences among different chemical treatments.Compared with the control,the germination rate and germination potential increased by 11.94%and16.55%,the radicle and hypocotyl elongated by 3.50 cm and 2.48 cm.The dry and fresh weight improved by 1.58 g and 2.79 g respectively,which were significantly different from the control(P<0.05).

关 键 词：黑芸豆 药剂处理 高通量测序 表面细菌群落 萌发指标 

分 类 号：S64[农业科学—蔬菜学]