脂滴特异性荧光探针的构建及可视化肝肿瘤细胞  

Construction of a Lipid Droplets Targeted Fluorescent Probe for Visualization of Liver Tumor Cells

作　　者：徐冬青 童海姗 沈杰 邱万伟 钱立生 Xu Dongqing;Tong Haishan;Shen Jie;Qiu Wanwei;Qian Lisheng(School of Life and Health Science,Anhui Science and Technology University,Fengyang,Anhui 233100)

机构地区：[1]安徽科技学院生命与健康科学学院,安徽凤阳233100

出　　处：《有机化学》2024年第4期1240-1246,共7页Chinese Journal of Organic Chemistry

基　　金：安徽省教育厅重点项目(Nos.2023AH051883,2022AH051632);大连金点生物科技有限公司资助的横向项目(No.880988);上海勍臻检测技术有限公司(No.881242)资助项目。

摘　　要：脂滴(LDs)参与了众多活细胞的代谢过程,其异常与多种疾病如脂肪肝、糖尿病和癌症等高度相关,但是准确阐明各种疾病与脂滴之间的相互关系仍然是一个巨大的挑战,尤其是借助异常分布的LDs实现对肝肿瘤的可视化研究报道较少.因此,本论文以三苯胺为供电子基团,多氰基杂环结构为电子受体,通过简单的两步反应合成了一种具有LDs特异性的荧光探针TCN-Tph.制备的探针分子TCN-Tph与脂滴的共定位成像实验表明,探针TCN-Tph对肝肿瘤细胞具有良好的LDs靶向能力.采用不同细胞株对探针分子TCN-Tph的响应实验结果表明,肝肿瘤细胞对其有很好的响应和摄取能力.不同状态的肝组织切片成像结果进一步表明,制备的探针分子TCN-Tph对正常肝组织和肝肿瘤组织具有良好的区分功能,为实现临床上肝肿瘤的早期预诊提供了实验基础.Lipid droplets(LDs)are involved in the metabolic processes of many living cells,and their anomalies are highly associated with a variety of diseases,such as fatty liver,diabetes and cancers.But it is still a great challenge to elucidate the intrinsic relationship between various diseases and LDs.In particular,there has been only a few studies on the visualization of abnormal LDs in liver tumor cells.Therefore,in this paper,a lipid droplets targeted fluorescent probe was easily prepared through a two-step procedure by using triphenylamine as the electron donor and polycyanide heterocyclic compound as the electron acceptor.The fluorescent probe TCN-Tph possesses good ability to target LDs in living cells through their co-localization imaging experiments.In comparison with other carcinoma cells,the liver tumor cells have good response and uptake of TCN-Tph.The slices images of liver tissues in different states further show that the probe TCN-Tph can also distinguish liver tissues and normal liver tissues effectively,which provide an experimental basis for the early diagnosis of liver tumor cells in clinic.

关 键 词：脂滴 荧光探针 肝肿瘤细胞 可视化 

分 类 号：R735.7[医药卫生—肿瘤] O657.3[医药卫生—临床医学]