RebL1 is required for macronuclear structure stability and gametogenesis in Tetrahymena thermophila  

作　　者：Huijuan Hao Yinjie Lian Chenhui Ren Sitong Yang Min Zhao Tao Bo Jing Xu Wei Wang 

机构地区：[1]Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Institute of Biotechnology,Shanxi University,Taiyuan 030006,China [2]School of Life Science,Shanxi University,Taiyuan 030006,China [3]Shanxi Key Laboratory of Biotechnology,Taiyuan 030006,China

出　　处：《Marine Life Science & Technology》2024年第2期183-197,共15页海洋生命科学与技术（英文）

基　　金：supported by the National Natural Science Foundation of China(32270450,32071449);Shanxi Key Program International S&T Cooperation Projects(202104041101011);Shanxi Scholarship Council of China(2020016).

摘　　要：Histone modification and nucleosome assembly play important roles in chromatin-related processes.Histone chaperones form different complexes and coordinate histone transportation and assembly.Various histone chaperone complexes have been identified in different organisms.The ciliate protozoa(ciliates)have various chromatin structures and different nuclear morphology.However,histone chaperone components and functions of different subunits remain unclear in ciliates.Tet-rahymema thermophila contains a transcriptionally active macronucleus(MAC)and a transcriptionally inactive micronu-cleus(MIC)which exhibit multiple replication and various chromatin remodeling progresses during vegetative growth and sexual developmental stages.Here,we found histone chaperone RebL1 not only localized evenly in the transcriptionally active MAC but also dynamically changed in the MIC during vegetative growth and sexual developmental stages.REBL1 knockdown inhibited cellular proliferation.The macronuclear morphology became bigger in growing mutants.The abnormal macronuclear structure also occurred in the starvation stage.Furthermore,micronuclear meiosis was disturbed during sexual development,leading to a failure to generate new gametic nuclei.RebL1 potentially interacted with various factors involved in histone-modifying complexes and chromatin remodeling complexes in different developmental stages.REBL1 knockdown affected expression levels of the genes involved in chromatin organization and transcription.Taken together,RebL1 plays a vital role in maintaining macronuclear structure stability and gametogenesis in T.thermophila.

关 键 词：GAMETOGENESIS Histone chaperone Macronuclear structure RebL1 Tetrahymena thermophilaIntroduction 

分 类 号：O62[理学—有机化学]