Combined proteomic and metabolomic studies on the liver of Amur sturgeon Acipenser schrenckii under titanium dioxide nanoparticle exposure  

作　　者：Zhou ZHOU Ting LIU Jie KONG Zhenxing ZHAO Junquan ZHU 

机构地区：[1]Key Laboratory of Aquacultural Biotechnology,Ministry of Education,and Key Laboratory of Marine Biotechnology of Zhejiang Province,College of Marine Sciences,Ningbo University,Ningbo 315000,China [2]Guizhou Fisheries Research Institute,Guizhou Academy of Agriculture Sciences,Guiyang 550000,China

出　　处：《Journal of Oceanology and Limnology》2024年第3期1001-1015,共15页海洋湖沼学报（英文）

基　　金：Supported by the Guizhou Provincial Basic Research Program(No.ZK[2023]175);the Earmarked Fund for the China Agriculture Research System(No.CARS-46)。

摘　　要：Nanomaterials,particularly titanium dioxide nanoparticles(TiO_(2)-NPs),are extensively utilized across various industries.However,their environmental release has raised concerns regarding their potential ecological and environmental impacts.The reproductive toxicity of TiO_(2)-NPs in fish species has attracted considerable attention,yet conflicting research outcomes have been reported.We investigated the effects of TiO_(2)-NPs exposure on the liver of juvenile Amur sturgeon Acipenser schrenckii using label-free proteomic and untargeted metabolomic analyses.The experiment included a control group and three groups exposed to different concentrations of TiO_(2)-NPs(low,TL;medium,TM;high,TH).Compared to the control group,9,19,and 25 proteins and 35,73,and 158 metabolites were differentially expressed in the TH,TM,and TL TiO_(2)-NP-exposed groups,respectively.The differentially expressed genes(DEGs)were enriched in the Kyoto Encyclopedia for Genes and Genomes(KEGG)pathways related to glycolysis and gluconeogenesis.Moreover,among the 126 correlated proteins,the most enriched pathways were associated with endocytosis and protein processing in the endoplasmic reticulum.Notably,syringic acid was significantly downregulated across all three TiO_(2)-NP-exposed groups.To obtain a comprehensive overview of the TiO_(2)-NP-induced expression changes,a co-regulated network of proteins and metabolites associated with TiO_(2)-NPs exposure was constructed.Exposure to TiO_(2)-NPs led to enrichment and alteration of pathways related to immune responses,including endocytosis,protein processing in the endoplasmic reticulum,and peroxisome proliferator-activated receptor(PPAR)signaling.In conclusion,our findings indicate that exposure to TiO_(2)-NPs might disrupt glucose metabolism and induce immune responses,thus contributing to our understanding of the environmental impacts of nanomaterials and highlighting the need for further research and development of potential mitigation strategies.

关 键 词：TiO_(2)-nanoparticle exposure proteomics metabolomics glucose metabolism immune response 

分 类 号：X174[环境科学与工程—环境科学]